Overview

  • Product name
    Anti-Ghrelin antibody (Biotin)
    See all Ghrelin primary antibodies
  • Description
    Rabbit polyclonal to Ghrelin (Biotin)
  • Host species
    Rabbit
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: ELISA, IP, RIA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cat, Dog, Pig, Baboon, Rhesus monkey, Gerbil
  • Immunogen

    Synthetic peptide: H-GSS(octanoyl)FLSPE HQRVQQRKES KKPPAKLQPR-OH conjugated to KLH via carboxyl group, corresponding to internal sequence amino acids 24-51 of Human Ghrelin.

  • Positive control
    • This antibody gave a positive result in IHC in the following FFPE tissue: Human normal stomach.

Properties

Applications

Our Abpromise guarantee covers the use of ab48285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
RIA Use a concentration of 2 - 8 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • Tissue specificity
    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • Sequence similarities
    Belongs to the motilin family.
  • Post-translational
    modifications
    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

Images

  • IHC image of Ghrelin staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48285, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab48285 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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Answer

Thank you for contacting Abcam regarding ab48285.


I am sorry that you have been experiencing difficulties with this antibody in your RIA experiments. I understand that you have tested a concentration of 4ug/ml - have you tested any others? We recommend up to 8ug/ml. Some further optimization may be required for best results with this antibody.


If increasing the amount of antibody used does not improve your results with this antibody, I am happy to offer a replacement or credit. Please do not hesitate to contact me if you have any additional questions.

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Answer

Thank you for contacting us.

The supplying lab sent us the following information:

The antibody isrecommended from 2-8 ug/ml for RIA purpose. The efficiency of labeling of their tracer is another factor to determine the concentration use for their RIA assay.The optimal condition would need to be determined by the enduser.

I am sorry that we did not receive more detailed information than this.
Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us. Please choose antibodies from the following catalogue numbers: anti Ghrelin ab48285, ab117620, ab15861, ab57222, ab104307 These antibodies are tested in Indirect ELISA so are guaranteed for this application and applications as stated on the datasheets. Anti Orexin ab35337, ab6214, ab16787, ab64565 These antibodies are tested in Indirect ELISA so are guaranteed for this application and applications as stated on the datasheets. Anti leptin ab20101, ab20115, ab9749 and ab2095 Anti Insulin ab6995, ab63820, ab7760 and ab46707 Anti neuropeptide Y We ab30914, ab10341, ab6173, ab43871, ab53478. These antibodies are not yet tested in ELISA. Anti peptide YY  ab51537, ab51539 etc. Anti ACTH  ab20358, ab21003, ab104768,a b104619 Anti MCH ab37337; These are however not yet tested in ELISA. Please check the datasheets of these antibodies for more up-to-date information. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

ab48285 has not been tested to determine if the antibody will bind Ghrelin whether it has octanoyl or no octanoyl.  Sorry this aspect of ab48285 has not yet been charactarized. If you have any other questions please feel free to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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