Recombinant
RabMAb

Recombinant Anti-Ghrelin antibody [EPR20502] (ab209790)

Overview

  • Product name

    Anti-Ghrelin antibody [EPR20502]
    See all Ghrelin primary antibodies
  • Description

    Rabbit monoclonal [EPR20502] to Ghrelin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ghrelin aa 1-100. The exact sequence is proprietary.
    Database link: Q9UBU3

  • Positive control

    • IHC-P: Human, mouse and rat stomach tissues. IHC-Fr: Mouse stomach tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209790 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/100.

Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20).

This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

Target

  • Function

    Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation.
    Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility.
  • Tissue specificity

    Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
  • Sequence similarities

    Belongs to the motilin family.
  • Post-translational
    modifications

    O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28-C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28-C10:1, may be modified with a singly unsaturated carboxylic acid.
    Amidation of Leu-98 is essential for obestatin activity.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Appetite regulating hormone antibody
    • Ghrelin 27 antibody
    • Ghrelin 28 antibody
    • Ghrelin and obestatin prepropeptide antibody
    • Ghrelin antibody
    • Ghrelin/obestatin prepropeptide antibody
    • ghrl antibody
    • GHRL_HUMAN antibody
    • Growth hormone releasing peptide antibody
    • Growth hormone secretagogue antibody
    • Growth hormone-releasing peptide antibody
    • M46 protein antibody
    • Motilin related peptide antibody
    • Motilin-related peptide antibody
    • MTLRP antibody
    • Obestatin antibody
    • Protein M46 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of human stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on endocrine cells of rat stomach is observed [PMID: 18474938]. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling Ghrelin with ab209790 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining on endocrine cells of mouse stomach is observed [PMID: 18474938]. The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This antibody worked well on mouse IHC-Frozen sections. Staining on rat sections was weak and human samples were not tested.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on human liver [PMID: 10604470].

    Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse liver [PMID: 10604470].

    Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ghrelin with ab209790 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on rat liver [PMID: 10604470].

    Counter stained with Hematoxylin.

References

This product has been referenced in:

  • Kawamura T  et al. Effects of Motilin Receptor Agonists and Ghrelin in Human motilin receptor Transgenic Mice. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30934667) »
See 1 Publication for this product

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