Overview

  • Product name
    Anti-Ghrelin O acyltransferase antibody
    See all Ghrelin O acyltransferase primary antibodies
  • Description
    Rabbit polyclonal to Ghrelin O acyltransferase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Ghrelin O acyltransferase aa 257-287 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q96T53

  • Positive control
    • MCF7 cell line and mouse stomach tissue lysates, Human pancreas tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 50 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Mediates the octanoylation of ghrelin at 'Ser-3'. Can use a variety of fatty acids as substrates including octanoic acid, decanoic acid and tetradecanoic acid.
  • Tissue specificity
    Expressed predominantly in stomach with moderate levels in pancreas and relatively low levels in most other tissues.
  • Sequence similarities
    Belongs to the membrane-bound acyltransferase family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FKSG89 antibody
    • ghrelin o transferas antibody
    • Ghrelin O-acyltransferase antibody
    • GOAT antibody
    • MBOA4_HUMAN antibody
    • Mboat4 antibody
    • Membrane-bound O-acyltransferase domain-containing protein 4 antibody
    • O-acyltransferase (membrane bound) domain containing 4 antibody
    • O-acyltransferase domain-containing protein 4 antibody
    • OACT4 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling Ghrelin O acyltransferase with ab170690 at 1/10 followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • All lanes : Anti-Ghrelin O acyltransferase antibody (ab170690) at 1/100 dilution

    Lane 1 : Mouse stomach tissue lysate
    Lane 2 : Mouse stomach tissue lysate with Blocking peptide

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size: 50 kDa

  • Anti-Ghrelin O acyltransferase antibody (ab170690) at 1/100 dilution + MCF7 cell line lysate at 35 µg

    Predicted band size: 50 kDa

References

ab170690 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Tissue lysate - whole (stomach)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
stomach
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Dec 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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