Anti-Ghrelin Receptor/GHS-R antibody (ab95250)

ab95259 Ghrelin Receptor/GHS-R antibody immunoreactivity in the mouse brain a.anterior hypothalamic nucleus b.high magnification immunoreactivity shows dendritic processes (arrowheads) and the periphery of the soma (arrows) of pyramidal cells in the somatosensory cortex c. hippocampus. The immunostaining with ab95250 is consistent with published data (Zigman et al, 2006).

ab95250 staining GHS-R in SKNSH cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with the antibody ab95250 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.