Overview

  • Product name

    Anti-GHRHR antibody - C-terminal
    See all GHRHR primary antibodies
  • Description

    Rabbit polyclonal to GHRHR - C-terminal
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Sheep, Human
    Predicted to work with: Mouse, Rat, Rabbit, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset
  • Immunogen

    Synthetic peptide, corresponding to 16 amino acids from the C terminus of Human GHRHR (NP_000814.2).

  • Positive control

    • Human anterior pituitary tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab150575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 16 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin embedded Human anterior pituitary tissue labeling GHRHR with ab150575 at 16ug/ml.
  • Immunofluorescence analysis of sheep anterior pituitary somatotropes, staining GHRHR with ab150575.

    Cells were fixed with formaldehyde and blocked with 20% serum for 2 hours at 20°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (16 µg/ml in 2% normal goat serum in TBS) for 2 hours at 25°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (2 µg/ml) was used as the secondary antibody.

    See Abreview

References

ab150575 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (Anterior Pituitary somatotropes)
Specification
Anterior Pituitary somatotropes
Fixative
Formaldehyde
Permeabilization
Yes - Boiling slides in 10mM sodium citrate solution, pH 6.0, for 10 minutes, then leaving to cool in the solution for 20 minutes.
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 05 2013

Answer

Thank you for your reply which has been forwarded to me as my colleague #### is currently away from the office.

I am very pleased to hear you would like to accept our offer and test ab150575 in sheep.

DISCOUNT CODE: ####
Valid from: ####
Expiration date: ####


Please note I have put the valid date as 1st January rather than todays date. So if you want to purchase before this date, please let me know so I can change the dates on the code. Codes are valid for 4 months.

This code will give you the value at which you purchased ab150575 off your next order before the expiration date. To redeem this offer, please submit an Abreview for sheep and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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Answer

Thank you for contacting us yesterday in regards to the anti-GHRHR antibody (ab150575).

Unfortunately, as you have discovered from the datasheet of this antibody, this antibody has not been used with sheep samples to our knowledge as yet. However, I have now checked the sequence homology shared between the immunogen used to raise the antibody and the sheep protein. This is 100% and we would therefore expect the antibody to cross react, however as we have not tested it we would not be able to guarantee this.

However, as discussed over the phone, if you would like to purchase ab150575 and use it with your sheep samples, we can offer you a testing discount. This offer means that if you purchase ab150575 as normal, test the antibody with your rabbit samples and let us know of the results through an Abreview (no matter whether positive or negative) you would then be eligible to claim the value of ab150575 (£227) off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/abtrial

Please note that the antibody to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab150575.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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