Lane order (L-R): HeLa, U2OS, 293T Full length giantin is approximately 370 kDa in size. This antibody failed to detect any obvious species of this Mw - although a series of high mw (>250 kDa) species were observed in all lysates, including HeLa - which express giantin as determined by ICC/IF. The upper range of the Mw markers is 250 kDa, therefore the mobility of these species is unclear. A strong band at ~200 kDa was observed in U2OS lysate, which is close to the predicted MW of a giantin transcript (variant c Aceview database).
This negative Abreview is currently being investigated by Abcam Technical Support. Please check here for an update response from Abcam.
Mr. Antibody Solutions
Submitted Jul 30 2008
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