Our purpose for using this antibody was for co-localization studies with the Golgi. However, we found that the antibody labelled the nucleus at a high dilution (1:20) and very little staining characteristic of the golgi was seen at either a high or low dilution. There may have been a very small subset of cells (1 in 300-400) that had possible perinuclear staining (which was predicted from other studies that used a different Giantin antibody in myoblasts). Upon inquiring with Abcam Technical Support, I was told that the portion of the protein this antibody was raised against has low homology between human and mouse, which likely explains the lack of golgi staining and off-target nuclear staining. I used 4% PFA fix, Triton permeabilization, BSA block, and AlexaFluor secondary.
Thank you for submitting an Abreview and you valuable feedback about the use of ab37266 in mouse. Based on the alignment of the immunogen and the mouse Golgb1 protein and your data, the antibody is most likely not suitable for mouse.
Abcam user community
Submitted Mar 01 2016
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