Immunocytochemistry/ Immunofluorescence abreview for Anti-Giantin antibody [9B6] - Golgi Marker

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®

Additional data

Additional Notes
This antibody was tested in both methanol and paraformaldehyde-fixed HeLa cells. Paraformaldehyde fixed cells gave much weaker and less specific staining patterns that required long exposure times. Methanol fixed cells gave a golgi staining pattern that was extremely bright and specific.

Dr. Kirk Mcmanus

Verified customer

Submitted Jun 29 2007

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