Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab174655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 376 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay participate in forming intercisternal cross-bridges of the Golgi complex.
Cellular localizationGolgi apparatus membrane.
- Information by UniProt
- 372 kDa Golgi complex associated protein antibody
- 372 kDa Golgi complex-associated protein antibody
- GCP antibody
All lanes : Anti-Giantin antibody (ab174655) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 376 kDa
Exposure time: 10 seconds
ab174655, at 1 µg/ml, labeling Giantin in 293T whole cell lysate immunoprecipitated using ab174655 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
ab174655 has not yet been referenced specifically in any publications.