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Abcam’s Giardia lamblia in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of Giardia lamblia antigens in faeces.
A 96-well plate has been precoated with polyclonal anti-Giardia lamblia antibodies. Controls or test samples are added to the wells and incubated; Giardia antigens present in the stool supernatant are captured. Following washing, a horseradish peroxidase (HRP) labelled anti-Giardia antibody is added to the wells, which binds to the immobilized antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Giardia lamblia antigen sample captured in plate.
|Components||1 x 96 tests|
|10X Wash Solution||1 x 100ml|
|Anti-Giardia lamblia Coated Microplate (12 x 8 well strips)||1 unit|
|Anti-Giardia lamblia Conjugate||1 x 12ml|
|Cover Foil||1 unit|
|Giardia lamblia Antigen Negative Control||1 x 2ml|
|Giardia lamblia Antigen Positive Control||1 x 2ml|
|Sample Diluent||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab178643 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
ab178643 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"