Key features and details
- Sample type: Tissue sections
Product nameGiemsa Stain Kit
Sample typeTissue sections
Giemsa Stain Kit (May-Grunwald) ab150670 is intended for use in the visualization of cells present in hematopoietic tissues and certain microorganisms. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.
Giemsa staining protocol summary:
- deparaffinize sections if necessary and rehydrate
- incubate with May-Grunwald solution for 5-7 min
- wash with PBS
- incubate with Giemsa solution for 10-15 min
- wash with PBS, and incubate in PBS for 5 min
- rinse with water and air dry
- clear with Xylene or similar, and mount
Other products for staining tissue sections
Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Nuclei Blue/Violet Cytoplasm Light Blue Collagen Pale Pink Muscle Fibre Pale Pink Erythrocytes Gray, Yellow or Pink Rickettsia Reddish-Purple Helicobacter Pylori Blue Mast Cells Dark Blue with Red Granules
Control Tissue: Blood Film. Any well fixed tissue.
Storage instructionsStore at room temperature. Please refer to protocols.
Components 100 tests Giemsa Stock Solution 1 x 500ml May Grunwald Stock Solution 1 x 500ml Phosphate Buffer solution (pH6.8) 1 x 500ml
ab150670 Giemsa Stain Kit staining formalin-fixed-paraffin embedded human bone marror.
ab150670 (Giemsa stain) staining mast cells (deep pink) in formalin fixed paraffin embedded normal human colon.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
Staining using ab150670 - Giemsa Stain Kit (May-Grunwald).
ab150670 has been referenced in 3 publications.
- Yen JH et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). PubMed: 30691497
- Li H et al. miR-23b promotes cutaneous wound healing through inhibition of the inflammatory responses by targeting ASK1. Acta Biochim Biophys Sin (Shanghai) 50:1104-1113 (2018). PubMed: 30188966
- Wu YX et al. CD146+ skeletal stem cells from growth plate exhibit specific chondrogenic differentiation capacity in vitro. Mol Med Rep 16:8019-8028 (2017). PubMed: 28983600