Overview

  • Product name
    Giemsa Stain Kit
  • Sample type
    Tissue sections
  • Product overview

    Giemsa Stain Kit (May-Grunwald) ab150670 is intended for use in the visualization of cells present in hematopoietic tissues and certain microorganisms. This kit may be used on formalin-fixed, paraffin-embedded or frozen sections.


    Giemsa staining protocol summary:
    - deparaffinize sections if necessary and rehydrate
    - incubate with May-Grunwald solution for 5-7 min
    - wash with PBS
    - incubate with Giemsa solution for 10-15 min
    - wash with PBS, and incubate in PBS for 5 min
    - rinse with water and air dry
    - clear with Xylene or similar, and mount


    Other products for staining tissue sections


    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

  • Notes

    Staining Interpretation

    Nuclei Blue/Violet
    Cytoplasm Light Blue
    Collagen Pale Pink
    Muscle Fibre Pale Pink
    Erythrocytes Gray, Yellow or Pink
    Rickettsia Reddish-Purple
    Helicobacter Pylori    Blue
    Mast Cells Dark Blue with Red Granules

    Control Tissue: Blood Film. Any well fixed tissue.

Properties

Images

  • ab150670 Giemsa Stain Kit staining formalin-fixed-paraffin embedded human bone marror.

  • ab150670 (Giemsa stain) staining mast cells (deep pink) in formalin fixed paraffin embedded normal human colon.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

  • Staining using ab150670 - Giemsa Stain Kit (May-Grunwald).

Protocols

References

This product has been referenced in:
  • Yen JH  et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). Read more (PubMed: 30691497) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

DPX – being a synthetic resin – should be ok to be used as a mounting media for this.

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