Product nameAnti-GIGYF1 antibody
See all GIGYF1 primary antibodies
DescriptionRabbit polyclonal to GIGYF1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8.
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab177509 was affinity purified using an epitope specific to GIGYF1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab177509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 115 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay act cooperatively with GRB10 to regulate tyrosine kinase receptor signaling. May increase IGF1 receptor phosphorylation under IGF1 stimulation as well as phosphorylation of IRS1 and SHC1.
Sequence similaritiesBelongs to the PERQ family.
Contains 1 GYF domain.
DomainThe GYF domain interacts with GRB10.
- Information by UniProt
- CDS2 antibody
- GIGYF1 antibody
- GRB10-interacting GYF protein 1 antibody
All lanes : Anti-GIGYF1 antibody (ab177509) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 115 kDa
Exposure time: 10 seconds
Detection of GIGYF1 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab177509 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab177509 was used at 0.4 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.
ab177509 has not yet been referenced specifically in any publications.