Anti-GILT antibody (ab232915)
Key features and details
- Rabbit polyclonal to GILT
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GILT antibody
See all GILT primary antibodies -
Description
Rabbit polyclonal to GILT -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein (His-T7-tag) corresponding to Human GILT aa 1 to the C-terminus. (Expressed in E.coli).
Database link: P13284 -
Positive control
- WB: Raji cell lysate; Recombinant human GILT protein. IHC-P: Human lung, prostate cancer, glioma, stomach, prostate and colorectal cancer tissues. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232915 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232915 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 28 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 28 kDa. |
Target
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Function
Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. -
Sequence similarities
Belongs to the GILT family. -
Post-translational
modificationsN-glycosylated. Sugar chains contain mannose-6-phosphate.
Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases. -
Cellular localization
Secreted. Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 10437 Human
- Omim: 604664 Human
- SwissProt: P13284 Human
- Unigene: 14623 Human
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Alternative names
- Gamma interferon inducible lysosomal thiol reductase antibody
- Gamma interferon inducible protein IP 30 antibody
- Gamma-interferon-inducible lysosomal thiol reductase antibody
see all
Images
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Anti-GILT antibody (ab232915) at 3 µg/ml + Recombinant human GILT protein
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa -
Anti-GILT antibody (ab232915) at 3 µg/ml + Raji (human Burkitt's lymphoma cell line) cell lysate
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa -
Paraffin-embedded human lung tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human prostate cancer stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human glioma tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human stomach tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human prostate tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human colorectal cancer tissue stained for GILT using ab232915 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for GILT (green) using ab232915 at 20 µg/ml in ICC/IF, followed by FITC staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab232915 has been referenced in 1 publication.
- Zhu C et al. IFI30 Is a Novel Immune-Related Target with Predicting Value of Prognosis and Treatment Response in Glioblastoma. Onco Targets Ther 13:1129-1143 (2020). PubMed: 32103982