Key features and details
- Mouse polyclonal to GILT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GILT antibody
See all GILT primary antibodies
DescriptionMouse polyclonal to GILT
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length protein corresponding to Human GILT aa 1-250.
- Human spleen lysate and transfected 293T lysate
This product was previously labelled as IFI30
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab70510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionLysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation.
Sequence similaritiesBelongs to the GILT family.
modificationsN-glycosylated. Sugar chains contain mannose-6-phosphate.
Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.
Cellular localizationSecreted. Lysosome.
- Information by UniProt
- Gamma interferon inducible lysosomal thiol reductase antibody
- Gamma interferon inducible protein IP 30 antibody
- Gamma-interferon-inducible lysosomal thiol reductase antibody
Anti-GILT antibody (ab70510) at 1/500 dilution + Human spleen tissue lysate at 25 µg
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 29 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
All lanes : Anti-GILT antibody (ab70510) at 1/500 dilution
Lane 1 : GILT transfected 293T lysate
Lane 2 : Non transfected 293T lysate
Lysates/proteins at 25 µg/ml per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
ab70510 has been referenced in 1 publication.
- Chen S et al. Lentivirus mediated ?-interferon-inducible lysosomal thiol reductase (GILT) knockdown suppresses human glioma U373MG cell proliferation. Biochem Biophys Res Commun 509:182-187 (2019). PubMed: 30587343