Overview

  • Product name

  • Description

    Mouse polyclonal to GILT
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human GILT aa 1-250.

  • Positive control

    • Human spleen lysate and transfected 293T lysate
  • General notes

     This product was previously labelled as IFI30

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation.
    • Sequence similarities

      Belongs to the GILT family.
    • Post-translational
      modifications

      N-glycosylated. Sugar chains contain mannose-6-phosphate.
      Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.
    • Cellular localization

      Secreted. Lysosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • Gamma interferon inducible lysosomal thiol reductase antibody
      • Gamma interferon inducible protein IP 30 antibody
      • Gamma-interferon-inducible lysosomal thiol reductase antibody
      • Gamma-interferon-inducible protein IP-30 antibody
      • GILT antibody
      • GILT_HUMAN antibody
      • IFI 30 antibody
      • IFI30 antibody
      • interferon gamma inducible protein 30 antibody
      • Interferon gamma inducible protein 30 preproprotein antibody
      • IP30 antibody
      • Legumaturain antibody
      • Lysosomal thiol reductase antibody
      • Lysosomal thiol reductase, gamma-interferon-inducible antibody
      see all

    Images

    • Anti-GILT antibody (ab70510) at 1/500 dilution + Human spleen tissue lysate at 25 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 29 kDa
      Observed band size: 27 kDa
      why is the actual band size different from the predicted?

    • All lanes : Anti-GILT antibody (ab70510) at 1/500 dilution

      Lane 1 : GILT transfected 293T lysate
      Lane 2 : Non transfected 293T lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 29 kDa
      Observed band size: 32 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Chen S  et al. Lentivirus mediated ?-interferon-inducible lysosomal thiol reductase (GILT) knockdown suppresses human glioma U373MG cell proliferation. Biochem Biophys Res Commun 509:182-187 (2019). Read more (PubMed: 30587343) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab70510.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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