Overview

  • Product name

  • Description

    Rabbit polyclonal to GILT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human GILT aa 14-250.

  • Positive control

    • WB:Raji cell lysate. IHC-P: AGS xenograft tissue.
  • General notes

     This product was previously labelled as IFI30

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.

Antigen retrieval: EDTA-based (pH 8.0), 15 minutes.

WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.

Target

  • Function

    Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (By similarity). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation.
  • Sequence similarities

    Belongs to the GILT family.
  • Post-translational
    modifications

    N-glycosylated. Sugar chains contain mannose-6-phosphate.
    Synthesized as a 35 kDa precursor which is then processed into the mature 30 kDa form via cleavage of N-terminal and C-terminal propeptides. Processing of the precursor is mediated by multiple lysosomal proteases.
  • Cellular localization

    Secreted. Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Gamma interferon inducible lysosomal thiol reductase antibody
    • Gamma interferon inducible protein IP 30 antibody
    • Gamma-interferon-inducible lysosomal thiol reductase antibody
    • Gamma-interferon-inducible protein IP-30 antibody
    • GILT antibody
    • GILT_HUMAN antibody
    • IFI 30 antibody
    • IFI30 antibody
    • interferon gamma inducible protein 30 antibody
    • Interferon gamma inducible protein 30 preproprotein antibody
    • IP30 antibody
    • Legumaturain antibody
    • Lysosomal thiol reductase antibody
    • Lysosomal thiol reductase, gamma-interferon-inducible antibody
    see all

Images

  • Anti-GILT antibody (ab96156) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 29 kDa



    12% SDS PAGE
  • Paraffin-embedded AGS xenograft tissue stained for GILT using ab96156 at 1/500 dilution in immunohistochemical analysis.

    Antigen retrieval: EDTA-based (pH 8.0), 15 minutes.

References

ab96156 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab96156.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up