Key features and details
- Mouse monoclonal [OTI1F11] to GilZ / TilZ
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-GilZ / TilZ antibody [OTI1F11]
See all GilZ / TilZ primary antibodies
DescriptionMouse monoclonal [OTI1F11] to GilZ / TilZ
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Orangutan
Recombinant full length protein corresponding to Human GilZ/ TilZ aa 1-134. (NP_004080) produced in HEK-293T cell.
MNTEMYQTPMEVAVYQLHNFSISFFSSLLGGDVVSVKLDNSASGASVVAI DNKIEQAMDLVKNHLMYAVREEVEILKEQIRELVEKNSQLERENTLLKTL ASPEQLEKFQSCLSPEEPAPESPQVPEAPGGSAV
Database link: Q99576
- WB: pCMV6-ENTRY GilZ / TilZ transfected HEK-293T cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 15 kDa.|
FunctionProtects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities.
Tissue specificityExpressed in brain, lung, spleen and skeletal muscle. Lower levels detected in heart and kidney. Not detected in the pancreas. In non-lymphoid tissues, in the absence of inflammation, the major source of constitutive expression is the macrophage lineage. Also expressed in cells from different hemopoietic cell lineages, including bone marrow cells, CD34+ stem cells, mature B- and T-cells, monocytes and granulocytes. Down-regulated in activated macrophages from inflammatory lesions of delayed-type hypersensitivity (DTH) reactions, such as in tuberculosis and in Crohn disease, whereas in Burkitt lymphoma, persists in macrophages involved in the phagocytosis of apoptotic malignant cells.
Sequence similaritiesBelongs to the TSC-22/Dip/Bun family.
DomainThe leucine-zipper is involved in homodimerization.
- Information by UniProt
- Delta sleep-inducing peptide immunoreactor antibody
- DIP antibody
- DSIP immunoreactive leucine zipper protein antibody
All lanes : Anti-GilZ / TilZ antibody [OTI1F11] (ab236524) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY GilZ / TilZ transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 15 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236524 has not yet been referenced specifically in any publications.