Key features and details
- Chicken polyclonal to GINS2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgY
Product nameAnti-GINS2 antibody
See all GINS2 primary antibodies
DescriptionChicken polyclonal to GINS2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human GINS2.
This product was previously labelled as PSF2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab37683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 21 kDa. We recommend using about 2.5 ug of positive control protein in the colorimetric method, and about 10x less for ECL detection.|
FunctionThe GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex seems to bind preferentially to single-stranded DNA.
Sequence similaritiesBelongs to the GINS2/PSF2 family.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- DNA replication complex GINS protein PSF2 antibody
- GINS complex subunit 2 (Psf2 homolog) antibody
- GINS complex subunit 2 antibody
Anti-GINS2 antibody (ab37683) at 1/2000 dilution + E coli derived fusion protein
Goat anti IgY-HRP at 1/1000 dilution
Predicted band size: 21 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
A fusion protien has been used in this western blot, which accounts for the differences in molecular weight, compared to the native protien.
ab37683 has been referenced in 3 publications.
- Tumini E et al. Physical and functional crosstalk between Fanconi anemia core components and the GINS replication complex. DNA Repair (Amst) 10:149-58 (2011). WB ; Human . PubMed: 21109493
- Cohen SM et al. BRG1 co-localizes with DNA replication factors and is required for efficient replication fork progression. Nucleic Acids Res 38:6906-19 (2010). ICC/IF ; Mouse . PubMed: 20571081
- Bauerschmidt C et al. Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases delta and epsilon during S phase. Genes Cells 12:745-58 (2007). PubMed: 17573775