Overview

  • Product name
    Anti-GIP antibody [04]
    See all GIP primary antibodies
  • Description
    Mouse monoclonal [04] to GIP
  • Host species
    Mouse
  • Specificity
    Ab30679 recognises GIP. It cross reacts up to 10% with PACAP and GLP2 but <0.1% with glucagon, GLP1 or VIP.
  • Tested applications
    Suitable for: IHC-P, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic human GIP coupled to carrier. The immunogen was adsorbed onto Al(OH)3 and emulsified in Freund's incomplete adjuvant.

  • General notes


    Fusion partner: SP2/mIL6.

Properties

Applications

Our Abpromise guarantee covers the use of ab30679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/10000.

This dilution guideline applies to glutaraldehyde linked GIP OA (molar ratio 4:1) coated at 1 mg/mL. With GIP coated directly at 2 µg/mL the dilution guideline is 1/2000.

IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
  • Sequence similarities
    Belongs to the glucagon family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gastric Inhibitory Peptide antibody
    • Gastric inhibitory polypeptide antibody
    • Gastric inhibitory polypeptide precursor antibody
    • GIP antibody
    • GIP_HUMAN antibody
    • Glucose dependent insulinotropic polypeptide antibody
    • Glucose-dependent insulinotropic polypeptide antibody
    • Incretin hormone antibody
    see all

Images

  • ab30679 at 1/100 staining GIP in human duodenum by IHC-P.

  • ab30679 at 1/100 staining GIP in human small intestine by IHC-P.

References

This product has been referenced in:
  • Lim DM  et al. Difference in protective effects of GIP and GLP-1 on endothelial cells according to cyclic adenosine monophosphate response. Exp Ther Med 13:2558-2564 (2017). Read more (PubMed: 28565879) »
  • Young RL  et al. Disordered control of intestinal sweet taste receptor expression and glucose absorption in type 2 diabetes. Diabetes 62:3532-41 (2013). Human . Read more (PubMed: 23761104) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (gut)
Specification
gut
Fixative
Paraformaldehyde
Permeabilization
Yes - Antigen retrieval
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Duodenum)
Specification
Duodenum
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
Goat Sera as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 24°C

Ms. Bridgette Chia

Verified customer

Submitted Aug 10 2010

Answer

Thank you for your enquiry regarding our product ab30679. I am sorry you have had difficulty obtaining satisfactory results from your binding experiments with this antibody. Although this antibody has been tested by ELISA, it has not been tested for this type of binding experiment, so I cannot guarantee it will work. However, I would like to make a couple of suggestions that I hope may help. Unfortunately, there are no details of the specific epitope this antibody recognises, but it could be that your I-125 label is within the antibody binding region of the protein, and therefore interfering with binding of the antibody. You may also like to check the pH of the solution you are binding in. Most antibodies bind more efficiently at pH7 to pH8. If you have problems performing the ELISA, please do get back to us and we will be very happy to offer technical support. You will need to fill in the following technical form and send it back to us: https://www.abcam.com/index.html?section=elisa&pageconfig=technical&mode=questionaire As requested, I have copied details of the laboratory ELISA testing procedure for this particular antibody below. I hope this helps. Good luck with your experiments. Please don't hesitate to get back in touch with us if you need to. ELISA PROCEDURE: ELISA, binding to directly immobilized GIP: Coat: GIP in 2 ug/mL Apply antibody 2nd layer: Simply start a 2-fold serial dilution from 1.0 ug/mL (Alternately apply ab30679 in concentrations of: 10 ng/mL - 20 ng/mL - 40 ng/mL - 150 ng/mL - 300 ng/mL - 400 ng/mL and 800 ng/mL) Detect bound ab30679 with HRP conjugated polyclonal anti-mouse Ig antibody Color development and stop solution applied according to local laboratory standard reagents) Inhibition ELISA, binding free GIP in solution Coat GIP in 2 ug/mL Inhibition of 0.1 ug/mL ab30679 to the GIP coat with free GIP in concentrations from 10 ng/mL - 2 ug/mL Detect bound antibody with HRP conjugated polyclonal anti-mouse Ig antibody Color development and stop solution applied according to local laboratory standard reagents.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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