Overview

  • Product name
  • Description
    Rabbit polyclonal to GIP
  • Host species
    Rabbit
  • Specificity
    ab22624 reacts with Glucose-dependent Insulinotropic Peptide (GIP).
  • Tested applications
    Suitable for: IHC-P, IHC-R, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Pig
  • Immunogen

    Full length native protein (purified) (Pig) conjugated to KLH by a CDI linker.

  • Positive control
    • Mammalian duodenal (K) endocrine cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab22624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-R 1/1000 - 1/8000. 1/1000 - 1/8000 (using method A) and 1/200-1/1200 (using method B) Dilute to working strength with phosphatebuffered saline (PBS) containing 0.1% w/v sodium azide. Method A: using overnight incubation and indirect immunoperoxidase procedures, such as PAP and ABCperoxidase. Method B: using overnight incubation and indirect immunofluorescence procedures.
Flow Cyt 1/150.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
  • Sequence similarities
    Belongs to the glucagon family.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gastric Inhibitory Peptide antibody
    • Gastric inhibitory polypeptide antibody
    • Gastric inhibitory polypeptide precursor antibody
    • GIP antibody
    • GIP_HUMAN antibody
    • Glucose dependent insulinotropic polypeptide antibody
    • Glucose-dependent insulinotropic polypeptide antibody
    • Incretin hormone antibody
    see all

References

This product has been referenced in:
  • Beumer J  et al. Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient. Nat Cell Biol 20:909-916 (2018). Read more (PubMed: 30038251) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Mouse Cell (jejunum)
Specification
jejunum
Preparation
Cell harvesting/tissue preparation method: Cell isolation was performed in PBS + EDTA 5mM + DTT 1mM
Sample buffer: RPMI 1640 + 10% FBS
Fixation
Paraformaldehyde
Permeabilization
Yes - PBS + SVF 2% + saponine 0,1%

Dr. Sandra Guilmeau

Verified customer

Submitted Jan 23 2012

Question
Answer

Code de remise : ********* Date d’expiration : JJ MM AAAA Merci pour votre appel et votre intéret pour les produits Abcam. Je suis ravi que vous souhaitiez participer à notre offre et tester ab22624 chez la souris L'homologie entre la GIP de porc (Swiss Prot P01281) utilisée comme immunogène et la GIP de souris (Swiss Prot P48756) est de 97.6%). Le code de réduction correspondant est *********. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire. Vous devez dans un premier temps soumettre une Abreview avec vos résultats chez la souris en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab22624. Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches. Termes et Conditions : www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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