Key features and details
- Rabbit polyclonal to GIP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GIP antibody
See all GIP primary antibodies
DescriptionRabbit polyclonal to GIP
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human GIP aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in Human Pancreas tissue lysate within western blot and human pancreas FFPE tissue sections.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPotent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion.
Sequence similaritiesBelongs to the glucagon family.
- Information by UniProt
- Gastric Inhibitory Peptide antibody
- Gastric inhibitory polypeptide antibody
- Gastric inhibitory polypeptide precursor antibody
Anti-GIP antibody (ab72167) at 1 µg/ml + Human pancreas tissue lysate - total protein (ab29816) at 10 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 17 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
The band observed at 15 kDa could potentially be a cleaved form of GIP due to the presence of a 21 amino acid signal peptide.
IHC image of ab72167 staining in human pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72167, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab72167 has not yet been referenced specifically in any publications.