Anti-GIP antibody (ab83177)

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Overview

  • Product name

    Anti-GIP antibody
    See all GIP primary antibodies

  • Description

    Rabbit polyclonal to GIP

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide corresponding to Human GIP aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab94377)

  • Positive control

    • Recombinant Human GIP protein (ab116416) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HEK293 whole cell; A498 whole cell; MDA-MB-361 whole cell; JEG-3 whole cell; Human Placenta Tissue

  • General notes

    Previously labelled as CTRP1.

Applications

Our Abpromise guarantee covers the use of ab83177 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Sequence similarities

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • C1q and tumor necrosis factor related protein 1 antibody
        • C1QT1_HUMAN antibody
        • C1QTNF1 antibody
        • Complement C1q tumor necrosis factor related protein 1 antibody
        • Complement C1q tumor necrosis factor-related protein 1 antibody
        • CTRP 1 antibody
        • CTRP1 antibody
        • FLJ90694 antibody
        • G protein coupled receptor interacting protein 1 antibody
        • G protein-coupled receptor-interacting protein antibody
        • GIP antibody
        • ZSIG37 antibody
        see all

      Images

      • Western blot - Anti-GIP antibody (ab83177)
        Western blot - Anti-GIP antibody (ab83177)
        All lanes : Anti-GIP antibody (ab83177) at 1 µg/ml

        Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
        Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
        Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
        Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
        Lane 5 : Human placenta tissue lysate - total protein (ab29745)

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 32 kDa
        Observed band size: 37 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.



        GIP contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

      References

      ab83177 has not yet been referenced specifically in any publications.

      Publishing research using ab83177? Please let us know so that we can cite the reference in this datasheet.

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