Anti-GIP antibody (ab83177)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-GIP antibody
See all GIP primary antibodies -
DescriptionRabbit polyclonal to GIP
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide corresponding to Human GIP aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94377) -
Positive control
- Recombinant Human GIP protein (ab116416) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HEK293 whole cell; A498 whole cell; MDA-MB-361 whole cell; JEG-3 whole cell; Human Placenta Tissue
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General notes
Previously labelled as CTRP1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab83177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Sequence similaritiesContains 1 C1q domain.
Contains 1 collagen-like domain. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 114897 Human
- GenBank: NP_940995 Human
- Omim: 610365 Human
- SwissProt: Q9BXJ1 Human
- Unigene: 201398 Human
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Alternative names
- C1q and tumor necrosis factor related protein 1 antibody
- C1QT1_HUMAN antibody
- C1QTNF1 antibody
see all
Images
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Western blot - Anti-GIP antibody (ab83177)All lanes : Anti-GIP antibody (ab83177) at 1 µg/ml
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 5 : Human placenta tissue lysate - total protein (ab29745)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.GIP contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
Datasheets and documents
References
ab83177 has not yet been referenced specifically in any publications.
