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Signal Transduction Metabolism Energy Metabolism
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Anti-GIP antibody (ab83177)

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Western blot - Anti-GIP antibody (ab83177)

    Key features and details

    • Rabbit polyclonal to GIP
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-GIP antibody
      See all GIP primary antibodies
    • Description

      Rabbit polyclonal to GIP
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human GIP aa 50-150 conjugated to keyhole limpet haemocyanin.
      (Peptide available as ab94377)

    • Positive control

      • Recombinant Human GIP protein (ab116416) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: HEK293 whole cell; A498 whole cell; MDA-MB-361 whole cell; JEG-3 whole cell; Human Placenta Tissue
    • General notes

      Previously labelled as CTRP1.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituent: PBS

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Cardiovascular
      • Atherosclerosis
      • Diabetes associated
      • Cardiovascular
      • Atherosclerosis
      • Vascular Inflammation
      • Inflammatory mediators
      • Cardiovascular
      • Lipids / Lipoproteins
      • Fatty Acids
      • Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Fatty acids
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism
      • Metabolism
      • Types of disease
      • Diabetes
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Fatty acid oxidation

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human GIP protein (ab151376)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab83177 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    Notes
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Sequence similarities

      Contains 1 C1q domain.
      Contains 1 collagen-like domain.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q9BXJ1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 114897 Human
      • GenBank: NP_940995 Human
      • Omim: 610365 Human
      • SwissProt: Q9BXJ1 Human
      • Unigene: 201398 Human
      • Alternative names

        • C1q and tumor necrosis factor related protein 1 antibody
        • C1QT1_HUMAN antibody
        • C1QTNF1 antibody
        • Complement C1q tumor necrosis factor related protein 1 antibody
        • Complement C1q tumor necrosis factor-related protein 1 antibody
        • CTRP 1 antibody
        • CTRP1 antibody
        • FLJ90694 antibody
        • G protein coupled receptor interacting protein 1 antibody
        • G protein-coupled receptor-interacting protein antibody
        • GIP antibody
        • ZSIG37 antibody
        see all

      Images

      • Western blot - Anti-GIP antibody (ab83177)
        Western blot - Anti-GIP antibody (ab83177)
        All lanes : Anti-GIP antibody (ab83177) at 1 µg/ml

        Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
        Lane 2 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
        Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate Whole Cell Lysate
        Lane 4 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
        Lane 5 : Human placenta tissue lysate - total protein (ab29745)

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Developed using the ECL technique.

        Performed under reducing conditions.

        Predicted band size: 32 kDa
        Observed band size: 37 kDa
        why is the actual band size different from the predicted?
        Additional bands at: 21 kDa. We are unsure as to the identity of these extra bands.



        GIP contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab83177? Please let us know so that we can cite the reference in this datasheet.

      ab83177 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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