Recombinant
RabMAb

Recombinant Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)

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Overview

  • Product name

    Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free
    See all GIP primary antibodies

  • Description

    Rabbit monoclonal [EPR20410] to GIP - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human GIP aa 50-150. The exact sequence is proprietary.
    Database link: P09681

  • Positive control

    • IHC-P: Human pancreas and duodenum tissues; Mouse pancreas and small intestine tissues; Rat pancreas tissue. IHC-Fr: Mouse pancreas tissue.

  • General notes

    ab223157 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab223157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling GIP with ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Cytoplasmic staining on alpha cells of human pancreas islet is observed (PMID: 4574902).

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209792).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)

    Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling GIP with ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Sporadic and cytoplasmic staining on human duodenum is observed (PMID: 4574902).

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209792).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling GIP with ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Cytoplasmic staining on alpha cells of mouse pancreas islet is observed (PMID: 4574902).

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209792).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling GIP with ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Sporadic and cytoplasmic staining on mouse small intestine is observed (PMID: 4574902).

    Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209792).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GIP with ab209792 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Cytoplasmic staining on alpha cells of rat pancreas islet is observed (PMID: 4574902).

    Counter stained with Hematoxylin. 

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209792).

  • Immunohistochemistry (Frozen sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)
    Immunohistochemistry (Frozen sections) - Anti-GIP antibody [EPR20410] - Low endotoxin, Azide free (ab223157)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling GIP with ab209792 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic staining on alpha cells of mouse pancreas islet is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab209792).

References

ab223157 has not yet been referenced specifically in any publications.

Publishing research using ab223157? Please let us know so that we can cite the reference in this datasheet.

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