• Product name
  • Description
    Goat polyclonal to GIPC1
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: GAIGDAKVGRY, corresponding to C terminal amino acids 323-333 of Human GIPC1.

  • Positive control
    • HeLa Lysate.
  • General notes
    GenBank Accession Number – NP_005707


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab5951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).Can be blocked with Human GIPC1 peptide (ab23130).
ICC/IF Use at an assay dependent dilution. PubMed: 20634288


  • Relevance
    GIPC1 is a hydrophilic protein that contains 333 amino acids, including multiple phosphorylation sites and an 80 to 100 amino acid PDZ domain. PDZ domain-containing proteins typically recognize C terminal amino acids and are involved in protein network signaling. GIPC1 may be involved in G protein-linked signaling.
  • Cellular localization
    Cell Membrane, peripheral membrane protein and Cytoplasmic.
  • Database links
  • Alternative names
    • C19ORF3 antibody
    • Chromosome 19 open reading frame 3 antibody
    • GAIP C-terminus-interacting protein antibody
    • GIPC antibody
    • GIPC PDZ domain containing family member 1 antibody
    • GLUT1 C terminal binding protein antibody
    • GLUT1CBP antibody
    • Hs.6454 antibody
    • IGF 1 receptor interacting protein 1 antibody
    • IIP 1 antibody
    • IIP1 antibody
    • NIP antibody
    • PDZ domain-containing protein GIPC1 antibody
    • Regulator of G protein signalling 19 interacting protein 1 antibody
    • RGS-GAIP-interacting protein antibody
    • RGS19 interacting protein 1 antibody
    • RGS19IP1 antibody
    • SEMCAP antibody
    • SYNECTIIN antibody
    • Tax interaction protein 2 antibody
    • TIP 2 antibody
    see all


  • ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL).
  • Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.

    Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size: 38 kDa
    Observed band size: 24,38 kDa
    why is the actual band size different from the predicted?

    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.

    See Abreview

  • ICC/IF image of ab5951 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5951, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Western blot
Rat Tissue lysate - whole (ventral striatum)
Gel Running Conditions
Non-reduced Denaturing (10% TGX)
Loading amount
30 µg
ventral striatum
Blocking step
licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 11 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (prostate tissue section)
prostate tissue section
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Arterial endothelial cells)
Loading amount
30 µg
Arterial endothelial cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Mrs. Elena Astanina

Verified customer

Submitted Apr 09 2008


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