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    gipc1-antibody-ab5951.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-GIPC1 antibody (ab5951)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (4)

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Western blot - Anti-GIPC1 antibody (ab5951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
  • Western blot - Anti-GIPC1 antibody (ab5951)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)

Key features and details

  • Goat polyclonal to GIPC1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

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Protein
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Recombinant Human GIPC1 protein (ab96769)

View more associated products

Overview

  • Product name

    Anti-GIPC1 antibody
  • Description

    Goat polyclonal to GIPC1
  • Host species

    Goat
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide: GAIGDAKVGRY, corresponding to C terminal amino acids 323-333 of Human GIPC1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    GenBank Accession Number – NP_005707

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Recombinant Protein

    • Recombinant Human GIPC1 protein (ab96769)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab5951 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
IHC-P (1)
Use at an assay dependent concentration.
WB (2)
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).

1 hour primary incubation is recommended for this product.

Notes
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 38 kDa).

1 hour primary incubation is recommended for this product.

Target

  • Relevance

    GIPC1 is a hydrophilic protein that contains 333 amino acids, including multiple phosphorylation sites and an 80 to 100 amino acid PDZ domain. PDZ domain-containing proteins typically recognize C terminal amino acids and are involved in protein network signaling. GIPC1 may be involved in G protein-linked signaling.
  • Cellular localization

    Cell Membrane, peripheral membrane protein and Cytoplasmic.
  • Database links

    • Entrez Gene: 10755 Human
    • Entrez Gene: 67903 Mouse
    • Entrez Gene: 83823 Rat
    • Omim: 605072 Human
    • SwissProt: O14908 Human
    • SwissProt: Q9Z0G0 Mouse
    • SwissProt: Q9Z254 Rat
    • Unigene: 655012 Human
    • Unigene: 20945 Mouse
    • Unigene: 30044 Rat
    see all
  • Alternative names

    • C19ORF3 antibody
    • Chromosome 19 open reading frame 3 antibody
    • GAIP C-terminus-interacting protein antibody
    • GIPC antibody
    • GIPC PDZ domain containing family member 1 antibody
    • GLUT1 C terminal binding protein antibody
    • GLUT1CBP antibody
    • Hs.6454 antibody
    • IGF 1 receptor interacting protein 1 antibody
    • IIP 1 antibody
    • IIP1 antibody
    • NIP antibody
    • PDZ domain-containing protein GIPC1 antibody
    • Regulator of G protein signalling 19 interacting protein 1 antibody
    • RGS-GAIP-interacting protein antibody
    • RGS19 interacting protein 1 antibody
    • RGS19IP1 antibody
    • SEMCAP antibody
    • SYNECTIIN antibody
    • Tax interaction protein 2 antibody
    • TIP 2 antibody
    see all

Images

  • Western blot - Anti-GIPC1 antibody (ab5951)
    Western blot - Anti-GIPC1 antibody (ab5951)
    ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL). ab5951 at 1µg/ml staining GIPC1 at approximately 40kDa in HeLa Lysate by Western blot (ECL).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
    Ab5951 (10ug/ml) staining human GIPC1 in human kidney by immunohistochemistry using paraffin embedded tissue.

    Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • Western blot - Anti-GIPC1 antibody (ab5951)
    Western blot - Anti-GIPC1 antibody (ab5951)
    Anti-GIPC1 antibody (ab5951) at 0.03 µg/ml + Human HeLa cell lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size: 38 kDa
    Observed band size: 24,38 kDa
    why is the actual band size different from the predicted?



    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIPC1 antibody (ab5951)This image is courtesy of an anonymous Abreview
    ab5951 staining GIPC1 in human prostate tissue section by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Cells were formalin fixed prior to blocking in 10% serum for 20 minutes at RT. The primary antibody was diluted 1/250 and incubated with the sample for 24 hour at 4°C. A biotin conjugated rabbit anti-goat antibody, diluted 1/250, was used as the secondary.

    See Abreview

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (4)

    Publishing research using ab5951? Please let us know so that we can cite the reference in this datasheet.

    ab5951 has been referenced in 4 publications.

    • Henkel B  et al. NHERF1 in Microvilli of Vomeronasal Sensory Neurons. Chem Senses 42:25-35 (2017). PubMed: 27655939
    • Wu D  et al. GIPC1 interacts with MyoGEF and promotes MDA-MB-231 breast cancer cell invasion. J Biol Chem 285:28643-50 (2010). ICC/IF ; Human . PubMed: 20634288
    • Valdembri D  et al. Neuropilin-1/GIPC1 signaling regulates alpha5beta1 integrin traffic and function in endothelial cells. PLoS Biol 7:e25 (2009). WB ; Human . PubMed: 19175293
    • Zerbinatti CV  et al. Oxysterol-binding protein-1 (OSBP1) modulates processing and trafficking of the amyloid precursor protein. Mol Neurodegener 3:5 (2008). WB ; Human . PubMed: 18348724

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-GIPC1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (ventral striatum)
    Gel Running Conditions
    Non-reduced Denaturing (10% TGX)
    Loading amount
    30 µg
    Specification
    ventral striatum
    Blocking step
    licor blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2µg/mL · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Western blot abreview for Anti-GIPC1 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Arterial endothelial cells)
    Loading amount
    30 µg
    Specification
    Arterial endothelial cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Mrs. Elena Astanina

    Verified customer

    Submitted Apr 09 2008

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