Overview

  • Product name

    Anti-GIPC3 antibody [EPR15315]
    See all GIPC3 primary antibodies
  • Description

    Rabbit monoclonal [EPR15315] to GIPC3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human GIPC3 aa 150-300. The exact sequence is proprietary.
    Database link: Q8TF64

  • Positive control

    • K562, HeLa and Human lymph node lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15315
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
IP 1/20 - 1/40.

Target

  • Relevance

    GIPC3 is widely expressed in adult and fetal tissues. Highest levels are found in jejunum, lymph node, parietal lobe, fetal spleen and fetal thymus. It is expressed in cervical, melanoma, chronic myelogenous and gastric cancer cell lines.
  • Database links

  • Alternative names

    • C19orf64 antibody
    • DFNB15 antibody
    • DFNB72 antibody
    • DFNB95 antibody
    • DKFZp686J1198 antibody
    • FLJ40925 antibody
    • GIPC 3 antibody
    • GIPC PDZ domain containing family member 3 antibody
    • PDZ domain containing protein GIPC 3 antibody
    • PDZ domain containing protein GIPC3 antibody
    • PDZ domain protein GIPC 3 antibody
    • PDZ domain protein GIPC3 antibody
    see all

Images

  • All lanes : Anti-GIPC3 antibody [EPR15315] (ab186426) at 1/10000 dilution

    Lane 1 : K562 lysate
    Lane 2 : Human lymph node lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/10000 dilution

    Predicted band size: 34 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Anti-GIPC3 antibody [EPR15315] (ab186426) at 1/50000 dilution + HeLa cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Western blot analysis of GIPC3 in HeLa cell lysate immunoprecipitated using ab186426 at 1/50 dilution (Lane 1). Lane 2: PBS instead of HeLa lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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