Anti-GIRK-3 antibody (ab134807)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-GIRK-3 antibody -
Description
Rabbit polyclonal to GIRK-3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human GIRK-3 (internal sequence).
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Positive control
- IHC-P: Human Brain, Cortex Neurons tissue; WB: LOVO cell extracts.
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General notes
Previously labelled as KCNJ9.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS (without Mg2+, Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab134807 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 44 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. -
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ9 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3765 Human
- Entrez Gene: 16524 Mouse
- Entrez Gene: 116560 Rat
- Omim: 600932 Human
- SwissProt: Q92806 Human
- SwissProt: P48543 Mouse
- SwissProt: Q63511 Rat
- Unigene: 66726 Human
see all -
Alternative names
- G protein activated inward rectifier potassium channel 3 antibody
- G protein coupled inward rectifier potassium channel antibody
- G protein-activated inward rectifier potassium channel 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK-3 antibody (ab134807)Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded (IHC-P) Human Brain, Cortex Neurons tissue labelling GIRK-3 with ab134807 at 5 µg/ml.
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Western blot - Anti-GIRK-3 antibody (ab134807)All lanes : Anti-GIRK-3 antibody (ab134807) at 1/500 dilution
Lane 1 : Protein extract from Lovo cells
Lane 2 : Protein extract from Lovo cells, treated with the synthesized peptide
Predicted band size: 44 kDa
Datasheets and documents
References
ab134807 has not yet been referenced specifically in any publications.
