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    girk-3-antibody-ab134807.pdf

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Neuroscience Neurotransmission Receptors / Channels Potassium Channels
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Anti-GIRK-3 antibody (ab134807)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK-3 antibody (ab134807)
  • Western blot - Anti-GIRK-3 antibody (ab134807)

Key features and details

  • Rabbit polyclonal to GIRK-3
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-GIRK-3 antibody
  • Description

    Rabbit polyclonal to GIRK-3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human GIRK-3 (internal sequence).

  • Positive control

    • IHC-P: Human Brain, Cortex Neurons tissue; WB: LOVO cell extracts.
  • General notes

    Previously labelled as KCNJ9. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS (without Mg2+, Ca2+)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Potassium Channels
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab134807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
  • Sequence similarities

    Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ9 subfamily.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q92806 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3765 Human
    • Entrez Gene: 16524 Mouse
    • Entrez Gene: 116560 Rat
    • Omim: 600932 Human
    • SwissProt: Q92806 Human
    • SwissProt: P48543 Mouse
    • SwissProt: Q63511 Rat
    • Unigene: 66726 Human
    • Unigene: 261168 Mouse
    see all
  • Alternative names

    • G protein activated inward rectifier potassium channel 3 antibody
    • G protein coupled inward rectifier potassium channel antibody
    • G protein-activated inward rectifier potassium channel 3 antibody
    • GIRK-3 antibody
    • GIRK3 antibody
    • Inward rectifier K(+) channel Kir3.3 antibody
    • Inwardly rectifier K(+) channel Kir3.3 antibody
    • Inwardly rectifier K+ channel KIR3.3 antibody
    • inwardly rectifying subfamily J member 9 antibody
    • IRK9_HUMAN antibody
    • Kcnj9 antibody
    • KIR3.3 antibody
    • Potassium channel antibody
    • Potassium channel inwardly rectifying subfamily J member 9 antibody
    • Potassium inwardly rectifying channel subfamily J member 9 antibody
    • Potassium inwardly rectifying channel subfamily J9 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK-3 antibody (ab134807)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIRK-3 antibody (ab134807)

    Immunohistochemistry analysis of Formalin fixed and Paraffin Embedded (IHC-P) Human Brain, Cortex Neurons tissue labelling GIRK-3 with ab134807 at 5 µg/ml.

  • Western blot - Anti-GIRK-3 antibody (ab134807)
    Western blot - Anti-GIRK-3 antibody (ab134807)
    All lanes : Anti-GIRK-3 antibody (ab134807) at 1/500 dilution

    Lane 1 : Protein extract from Lovo cells
    Lane 2 : Protein extract from Lovo cells, treated with the synthesized peptide

    Predicted band size: 44 kDa

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab134807? Please let us know so that we can cite the reference in this datasheet.

ab134807 has not yet been referenced specifically in any publications.

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