Product nameAnti-GIRK1 antibody [EPR6363]
See all GIRK1 primary antibodies
DescriptionRabbit monoclonal [EPR6363] to GIRK1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in the extracellular domain of Human GIRK1. (Uniprot: P48549)
- HeLa, Fetal heart and NIH/3T3 cell lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 8.50 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionThis potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.
Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.
- Information by UniProt
- G protein activated inward rectifier potassium channel 1 antibody
- G protein-activated inward rectifier potassium channel 1 antibody
- GIRK-1 antibody
All lanes : Anti-GIRK1 antibody [EPR6363] (ab129182) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal heart cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Flow cytometric analysis using ab129182, at 1/100 dilution, staining GIRK1 in NIH/3T3 cells (red). Isotype control antibody (green).
Equilibrium disassociation constant (KD)
Learn more about KD
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This product has been referenced in:
- Binas S et al. miR-221 and -222 target CACNA1C and KCNJ5 leading to altered cardiac ion channel expression and current density. Cell Mol Life Sci N/A:N/A (2019). Read more (PubMed: 31312877) »
- May LM et al. G-Protein-Coupled Inwardly Rectifying Potassium (GIRK) Channel Activation by the p75 Neurotrophin Receptor Is Required for Amyloid ß Toxicity. Front Neurosci 11:455 (2017). Read more (PubMed: 28848381) »