Overview

  • Product name

    Anti-GIRK1 antibody [EPR6363]
    See all GIRK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR6363] to GIRK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the extracellular domain of Human GIRK1. (Uniprot: P48549)

  • Positive control

    • HeLa, Fetal heart and NIH/3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.
    • Sequence similarities

      Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • G protein activated inward rectifier potassium channel 1 antibody
      • G protein-activated inward rectifier potassium channel 1 antibody
      • GIRK-1 antibody
      • GIRK1 antibody
      • Inward rectifier K(+) channel Kir3.1 antibody
      • Inwardly rectifying potassium channel KIR3.1 antibody
      • inwardly rectifying subfamily J member 3 antibody
      • IRK3 antibody
      • IRK3_HUMAN antibody
      • Kcnf3 antibody
      • Kcnj3 antibody
      • KGA antibody
      • KGB1 antibody
      • Kir3.1 antibody
      • Potassium channel antibody
      • Potassium channel, inwardly rectifying subfamily J member 3 antibody
      • Potassium inwardly-rectifying channel, subfamily J, member 3 antibody
      see all

    Images

    • All lanes : Anti-GIRK1 antibody [EPR6363] (ab129182) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Fetal heart cell lysate
      Lane 3 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-rabbit HRP

      Predicted band size: 57 kDa
      Observed band size: 55 kDa
      why is the actual band size different from the predicted?

    • Flow cytometric analysis using ab129182, at 1/100 dilution, staining GIRK1 in NIH/3T3 cells (red). Isotype control antibody (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • May LM  et al. G-Protein-Coupled Inwardly Rectifying Potassium (GIRK) Channel Activation by the p75 Neurotrophin Receptor Is Required for Amyloid ß Toxicity. Front Neurosci 11:455 (2017). Read more (PubMed: 28848381) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Western blot
    Sample
    Mouse Tissue lysate - other (mouse brain lysate - post nuclear supernatant)
    Loading amount
    20 µg
    Specification
    mouse brain lysate - post nuclear supernatant
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Mar 04 2013

    Application
    Western blot
    Sample
    Human Cell lysate - other (HeLa lysate - post nuclear supernatant)
    Loading amount
    20 µg
    Specification
    HeLa lysate - post nuclear supernatant
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

    Abcam user community

    Verified customer

    Submitted Mar 04 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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