Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6363] to GIRK1 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-GIRK1 antibody [EPR6363] - BSA and Azide free
See all GIRK1 primary antibodies
DescriptionRabbit monoclonal [EPR6363] to GIRK1 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, WBmore details
Unsuitable for: ICC,ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human GIRK1 aa 100-200 (extracellular). The exact sequence is proprietary.
Database link: P48549
Ab176720 is the carrier-free version of ab129182. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab176720 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 8.50 x 10 -11 M Learn more about KD
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab176720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 57 kDa).|
FunctionThis potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. This receptor plays a crucial role in regulating the heartbeat.
Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ3 subfamily.
- Information by UniProt
- G protein activated inward rectifier potassium channel 1 antibody
- G protein-activated inward rectifier potassium channel 1 antibody
- GIRK-1 antibody
All lanes : Anti-GIRK1 antibody [EPR6363] (ab129182) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Fetal heart tissue lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-rabbit HRP
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
This data was developed using ab129182, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab176720 has not yet been referenced specifically in any publications.