Anti-GIRK2 antibody (ab65096)
Key features and details
- Goat polyclonal to GIRK2
- Suitable for: ELISA, IHC-P, WB, ICC/IF, IHC-FrFl
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-GIRK2 antibody
See all GIRK2 primary antibodies -
Description
Goat polyclonal to GIRK2 -
Host species
Goat -
Tested applications
Suitable for: ELISA, IHC-P, WB, ICC/IF, IHC-FrFlmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Syrian hamster, Orangutan -
Immunogen
Synthetic peptide within Human GIRK2 aa 350 to the C-terminus (Cysteine residue). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: Human Brain (Substantia Nigra) tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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WB |
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa).
1 hour primary incubation is recommended for this product. |
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ICC/IF |
Use at an assay dependent concentration. PubMed: 24586273
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IHC-FrFl | (1) |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 2 - 6 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 47 kDa). 1 hour primary incubation is recommended for this product. |
ICC/IF
Use at an assay dependent concentration. PubMed: 24586273 |
IHC-FrFl
Use at an assay dependent concentration. |
Target
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Function
This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. -
Tissue specificity
Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas. -
Involvement in disease
Keppen-Lubinsky syndrome -
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3763 Human
- Entrez Gene: 16522 Mouse
- Entrez Gene: 100174134 Orangutan
- Entrez Gene: 25743 Rat
- Omim: 600877 Human
- SwissProt: P48051 Human
- SwissProt: P48542 Mouse
- SwissProt: P48550 Rat
see all -
Alternative names
- inwardly rectifying subfamily J member 6 antibody
- BIR1 antibody
- G protein activated inward rectifier potassium channel 2 antibody
see all
Images
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Anti-GIRK2 antibody (ab65096) at 0.5 µg/ml + Human Brain (Substantia Nigra) tissue lysate in RIPA buffer at 35 µg
Predicted band size: 47 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?Primary incubation for 1 hour. Detected by chemiluminescence.
Protocols
Datasheets and documents
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Datasheet download
References (9)
ab65096 has been referenced in 9 publications.
- Gilmozzi V et al. Generation of hiPSC-Derived Functional Dopaminergic Neurons in Alginate-Based 3D Culture. Front Cell Dev Biol 9:708389 (2021). PubMed: 34409038
- Xiong M et al. Human Stem Cell-Derived Neurons Repair Circuits and Restore Neural Function. Cell Stem Cell 28:112-126.e6 (2021). PubMed: 32966778
- Chabrat A et al. Pharmacological Transdifferentiation of Human Nasal Olfactory Stem Cells into Dopaminergic Neurons. Stem Cells Int 2019:2945435 (2019). PubMed: 31236114
- Ahmed M et al. Laminin a2 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development. Development 146:N/A (2019). PubMed: 31371375
- Kim H et al. Modeling G2019S-LRRK2 Sporadic Parkinson's Disease in 3D Midbrain Organoids. Stem Cell Reports 12:518-531 (2019). PubMed: 30799274
- Jovanovic VM et al. BMP/SMAD Pathway Promotes Neurogenesis of Midbrain Dopaminergic Neurons In Vivo and in Human Induced Pluripotent and Neural Stem Cells. J Neurosci 38:1662-1676 (2018). PubMed: 29321139
- Sommer A et al. Th17 Lymphocytes Induce Neuronal Cell Death in a Human iPSC-Based Model of Parkinson's Disease. Cell Stem Cell 23:123-131.e6 (2018). PubMed: 29979986
- Zhao Z et al. Effects of Feeder Cells on Dopaminergic Differentiation of Human Embryonic Stem Cells. Front Cell Neurosci 10:291 (2016). ICC . PubMed: 28066186
- Hartfield EM et al. Physiological characterisation of human iPS-derived dopaminergic neurons. PLoS One 9:e87388 (2014). ICC/IF ; Human . PubMed: 24586273