Recombinant Anti-GIRK2 antibody [EPR22347-2] (ab229913)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22347-2] to GIRK2
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-GIRK2 antibody [EPR22347-2]
See all GIRK2 primary antibodies -
Description
Rabbit monoclonal [EPR22347-2] to GIRK2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details
Unsuitable for: IHC-Fr or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and liver tissue lysate; rat brain and liver tissue lysate IP: Mouse hippocampus lysate; rat brain lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22347-2 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
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Notes |
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IP
1/30. |
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa). |
Target
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Function
This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. -
Tissue specificity
Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas. -
Involvement in disease
Keppen-Lubinsky syndrome -
Sequence similarities
Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16522 Mouse
- Entrez Gene: 25743 Rat
- SwissProt: P48542 Mouse
- SwissProt: P48550 Rat
- Unigene: 328720 Mouse
- Unigene: 10185 Rat
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Alternative names
- inwardly rectifying subfamily J member 6 antibody
- BIR1 antibody
- G protein activated inward rectifier potassium channel 2 antibody
see all
Images
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All lanes : Anti-GIRK2 antibody [EPR22347-2] (ab229913) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 10 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Negative control: Liver tissue (PMID: 8929423)
This antibody reacts with a undetermined protein near 90KD.
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GIRK2 was immunoprecipitated from 0.35mg of rat brain whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: rat brain lysate (10μg) (Input).
Lane 2: ab229913 IP in rat brain lysate (Input).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in rat brain lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 7 seconds
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GIRK2 was immunoprecipitated from 0.35mg of mouse hippocampus whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: Mouse hippocampus lysate (10μg) (Input).
Lane 2: ab229913 IP in mouse hippocampus lysate (Input).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in Mouse hippocampus lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab229913 has not yet been referenced specifically in any publications.