Product nameAnti-GIRK2 antibody [EPR22347-2]
See all GIRK2 primary antibodies
DescriptionRabbit monoclonal [EPR22347-2] to GIRK2
Tested applicationsSuitable for: IP, WBmore details
Unsuitable for: IHC-Fr or IHC-P
Species reactivityReacts with: Mouse, Rat
Recombinant fragment within Mouse GIRK2 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: P48542
- WB: Mouse brain and liver tissue lysate; rat brain and liver tissue lysate IP: Mouse hippocampus lysate; rat brain lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab229913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).|
FunctionThis potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
Tissue specificityMost abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.
Involvement in diseaseKeppen-Lubinsky syndrome
Sequence similaritiesBelongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily.
- Information by UniProt
- inwardly rectifying subfamily J member 6 antibody
- BIR1 antibody
- G protein activated inward rectifier potassium channel 2 antibody
All lanes : Anti-GIRK2 antibody [EPR22347-2] (ab229913) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 10 seconds
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Negative control: Liver tissue (PMID: 8929423)
This antibody reacts with a undetermined protein near 90KD.
GIRK2 was immunoprecipitated from 0.35mg of rat brain whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: rat brain lysate (10μg) (Input).
Lane 2: ab229913 IP in rat brain lysate (Input).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in rat brain lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST
Exposure time: 7 seconds
GIRK2 was immunoprecipitated from 0.35mg of mouse hippocampus whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.
Lane 1: Mouse hippocampus lysate (10μg) (Input).
Lane 2: ab229913 IP in mouse hippocampus lysate (Input).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in Mouse hippocampus lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7 seconds.
ab229913 has not yet been referenced specifically in any publications.