Overview

  • Product name

    Anti-GIRK2 antibody [EPR22347-2]
    See all GIRK2 primary antibodies
  • Description

    Rabbit monoclonal [EPR22347-2] to GIRK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: IHC-Fr or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse GIRK2 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P48542

  • Positive control

    • WB: Mouse brain and liver tissue lysate; rat brain and liver tissue lysate IP: Mouse hippocampus lysate; rat brain lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab229913 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Target

    • Function

      This potassium channel may be involved in the regulation of insulin secretion by glucose and/or neurotransmitters acting through G-protein-coupled receptors. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.
    • Tissue specificity

      Most abundant in cerebellum, and to a lesser degree in islets and exocrine pancreas.
    • Involvement in disease

      Keppen-Lubinsky syndrome
    • Sequence similarities

      Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ6 subfamily.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • inwardly rectifying subfamily J member 6 antibody
      • BIR1 antibody
      • G protein activated inward rectifier potassium channel 2 antibody
      • G protein-activated inward rectifier potassium channel 2 antibody
      • GIRK-2 antibody
      • GIRK2 antibody
      • hiGIRK2 antibody
      • Inward rectifier K(+) channel Kir3.2 antibody
      • Inward rectifier potassium channel KIR3.2 antibody
      • KATP-2 antibody
      • KATP2 antibody
      • KCNJ6 antibody
      • KCNJ6_HUMAN antibody
      • Kcnj7 antibody
      • KIR3.2 antibody
      • KPLBS antibody
      • Potassium channel antibody
      • Potassium channel inwardly rectifying subfamily J member 6 antibody
      • Potassium inwardly rectifying channel subfamily J member 6 antibody
      • Potassium voltage gated channel subfamily J member 6 antibody
      see all

    Images

    • All lanes : Anti-GIRK2 antibody [EPR22347-2] (ab229913) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : Rat brain tissue lysate
      Lane 4 : Rat liver tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Predicted band size: 48 kDa
      Observed band size: 48 kDa


      Exposure time: 10 seconds


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST

      Negative control: Liver tissue (PMID: 8929423)

      This antibody reacts with a undetermined protein near 90KD.

    • GIRK2 was immunoprecipitated from 0.35mg of rat brain whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

      Lane 1: rat brain lysate (10μg) (Input). 

      Lane 2: ab229913 IP in rat brain lysate  (Input). 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in rat brain lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST

      Exposure time: 7 seconds

    • GIRK2 was immunoprecipitated from 0.35mg of mouse hippocampus whole cell lysate with ab229913 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab229913 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.

      Lane 1: Mouse hippocampus lysate (10μg) (Input). 

      Lane 2: ab229913 IP in mouse hippocampus lysate  (Input). 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229913 in Mouse hippocampus lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 7 seconds.

    References

    ab229913 has not yet been referenced specifically in any publications.

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