Product nameAnti-GIT1 antibody
See all GIT1 primary antibodies
DescriptionRabbit polyclonal to GIT1
Tested applicationsSuitable for: IHC-P, IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Synthetic peptide within Human GIT1 aa 600-650. The exact sequence is proprietary. NP_054749.2
Database link: Q9Y2X7
- IHC-P: Human Breast Carcinoma tissue; IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
Our Abpromise guarantee covers the use of ab264273 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
|IP||Use at 2-5 µg/mg of lysate.|
FunctionGTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
Sequence similaritiesContains 3 ANK repeats.
Contains 1 Arf-GAP domain.
DomainThe paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
modificationsPhosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.
Cellular localizationCytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
- Information by UniProt
- ARF GAP GIT1 antibody
- ARF GTPase activating protein GIT1 antibody
- ARF GTPase-activating protein GIT1 antibody
GIT1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab264273 at 3 μg per reaction. Western blot was performed on the immunoprecipitates using adifferent GIT1 antibidt at 1 μg/ml.
Lane 1: ab264273 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in Hela whole cell lysate.
Exposure time: 30 seconds.
Immunohistochemical analysis of FFPE human breast carcinoma tissue section labelling GIT1 with ab264273 at 1/1000 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264273 has not yet been referenced specifically in any publications.