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    git1-antibody-ab86235.pdf

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Signal Transduction Adapters Cytoplasmic
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Anti-GIT1 antibody (ab86235)

  • Datasheet
  • SDS
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Western blot - Anti-GIT1 antibody (ab86235)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIT1 antibody (ab86235)
  • Immunoprecipitation - Anti-GIT1 antibody (ab86235)

Key features and details

  • Rabbit polyclonal to GIT1
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-GIT1 antibody
    See all GIT1 primary antibodies
  • Description

    Rabbit polyclonal to GIT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residue 375 and 425 of Human GIT1 (NP_054749.2).

  • Positive control

    • Whole cell lysate from HeLa cells and 293T cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab86235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP
Use at 2-5 µg/mg of lysate.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
  • Sequence similarities

    Contains 3 ANK repeats.
    Contains 1 Arf-GAP domain.
  • Domain

    The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.
  • Cellular localization

    Cytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
  • Target information above from: UniProt accession Q9Y2X7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 28964 Human
    • Omim: 608434 Human
    • SwissProt: Q9Y2X7 Human
    • Unigene: 514051 Human
    • Alternative names

      • ARF GAP GIT1 antibody
      • ARF GTPase activating protein GIT1 antibody
      • ARF GTPase-activating protein GIT1 antibody
      • CAT 1 antibody
      • CAT-1 antibody
      • CaT1 antibody
      • Cool associated and tyrosine phosphorylated protein 1 antibody
      • Cool-associated and tyrosine-phosphorylated protein 1 antibody
      • G protein coupled receptor kinase interacting ArfGAP 1 antibody
      • G protein coupled receptor kinase interactor 1 antibody
      • G protein-coupled receptor kinase-interactor 1 antibody
      • GIT1 antibody
      • GIT1_HUMAN antibody
      • GRK interacting protein 1 antibody
      • GRK-interacting protein 1 antibody
      see all

    Images

    • Western blot - Anti-GIT1 antibody (ab86235)
      Western blot - Anti-GIT1 antibody (ab86235)
      All lanes : Anti-GIT1 antibody (ab86235) at 0.04 µg/ml

      Lane 1 : Whole cell lysate from HeLa cells at 50 µg
      Lane 2 : Whole cell lysate from HeLa cells at 15 µg
      Lane 3 : Whole cell lysate from HeLa cells at 5 µg
      Lane 4 : Whole cell lysate from 293T cells at 50 µg

      Developed using the ECL technique.

      Predicted band size: 84 kDa
      Observed band size: 90 kDa why is the actual band size different from the predicted?
      Additional bands at: 275 kDa, 56 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 3 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIT1 antibody (ab86235)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIT1 antibody (ab86235)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling GIT1 with ab86235 at 1/200 (1µg/ml). Detection: DAB.
    • Immunoprecipitation - Anti-GIT1 antibody (ab86235)
      Immunoprecipitation - Anti-GIT1 antibody (ab86235)
      Detection of GIT1 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86235 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab86235? Please let us know so that we can cite the reference in this datasheet.

    ab86235 has not yet been referenced specifically in any publications.

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