Recombinant Anti-GIT1 antibody [EPR10367] (ab156001)
![Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)](https://www.abcam.com/ps/products/156/ab156001/Images/ab156001-412750-anti-git1-antibody-epr10367-western-blot-human-knockout-lysate.jpg "Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10367] to GIT1
- Suitable for: Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-GIT1 antibody [EPR10367]
See all GIT1 primary antibodies -
Description
Rabbit monoclonal [EPR10367] to GIT1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y, HeLa, 293T, brain nuclear extract and fetal brain lysates. Flow Cyt (intra): 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10367 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 84 kDa. |
Target
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Function
GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes. -
Sequence similarities
Contains 3 ANK repeats.
Contains 1 Arf-GAP domain. -
Domain
The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6. -
Post-translational
modificationsPhosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase. -
Cellular localization
Cytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin. - Information by UniProt
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Database links
- Entrez Gene: 28964 Human
- Entrez Gene: 216963 Mouse
- Entrez Gene: 83709 Rat
- Omim: 608434 Human
- SwissProt: Q9Y2X7 Human
- SwissProt: Q68FF6 Mouse
- SwissProt: Q9Z272 Rat
- Unigene: 514051 Human
see all -
Alternative names
- ARF GAP GIT1 antibody
- ARF GTPase activating protein GIT1 antibody
- ARF GTPase-activating protein GIT1 antibody
see all
Images
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Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : G1T1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Human brain tissue nuclear fraction lysate
Lane 4 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 84 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab156001 observed at 98 kDa. Red - loading control ab8245 observed at 36 kDa.
ab156001 Anti-GIT1 antibody [EPR10367] was shown to specifically react with GIT1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265230 (knockout cell lysate ab257965) was used. Wild-type and GIT1 knockout samples were subjected to SDS-PAGE. ab156001 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry (Intracellular) - Anti-GIT1 antibody [EPR10367] (ab156001)](https://www.abcam.com/ps/products/156/ab156001/Images/GIT1-Primary-antibodies-ab156001-2.jpg)
Flow Cytometry (Intracellular) - Anti-GIT1 antibody [EPR10367] (ab156001)Intracellular flow cytometric analysis of permeabilized 293T cells labeling GIT1 with ab156001 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).
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![Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)](https://www.abcam.com/ps/products/156/ab156001/Images/GIT1-Primary-antibodies-ab156001-1.jpg)
Western blot - Anti-GIT1 antibody [EPR10367] (ab156001)All lanes : Anti-GIT1 antibody [EPR10367] (ab156001) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 84 kDa -
![Anti-GIT1 antibody [EPR10367] (ab156001)](https://www.abcam.com/ps/products/156/ab156001/Images/ab156001-4-benefits-of-recombinant-antibodies.png)
Anti-GIT1 antibody [EPR10367] (ab156001)
Datasheets and documents
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Datasheet download
References (1)
ab156001 has been referenced in 1 publication.
- Sulimenko V et al. Microtubule nucleation in mouse bone marrow-derived mast cells is regulated by the concerted action of GIT1/ßPIX proteins and calcium. J Immunol 194:4099-111 (2015). PubMed: 25821222
