Recombinant
RabMAb

Anti-GIT1 antibody [EPR10368] (ab171956)

Overview

  • Product name
    Anti-GIT1 antibody [EPR10368]
    See all GIT1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10368] to GIT1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide within Human GIT1 aa 550-650. The exact sequence is proprietary.
    Database link: Q9Y2X7

  • Positive control
    • Human fetal brain, HeLa and SH-SY5Y lysates, paraffin-embedded Human pancreas tissue, paraffin-embedded Human thyroid carcinoma tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 84 kDa.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function
      GTPase-activating protein for the ADP ribosylation factor family. May serve as a scaffold to bring together molecules to form signaling modules controlling vesicle trafficking, adhesion and cytoskeletal organization. Increases the speed of cell migration, as well as the size and rate of formation of protrusions, possibly by targeting PAK1 to adhesions and the leading edge of lamellipodia. Sequesters inactive non-tyrosine-phosphorylated paxillin in cytoplasmic complexes.
    • Sequence similarities
      Contains 3 ANK repeats.
      Contains 1 Arf-GAP domain.
    • Domain
      The paxillin-binding domain is masked in the full-length protein and is regulated by ARHGEF6.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues by PTK2 and SRC in growing fibroblasts. Tyrosine-phosphorylation is increased following cell spreading on fibronectin, decreased in cells arrested in mitosis and increased in the ensuing G1 phase.
    • Cellular localization
      Cytoplasm. Cycles between at least 3 distinct intracellular compartments, including focal adhesions, cytoplasmic complexes and membrane protrusions. During cell migration, when cells detach, moves from the adhesions into the cytoplasmic complexes towards the leading edge, while, when cells adhere, it is found in vinculin-containing adhesions. Recruitment to adhesions may be mediated by active tyrosine-phosphorylated paxillin.
    • Information by UniProt
    • Database links
    • Alternative names
      • ARF GAP GIT1 antibody
      • ARF GTPase activating protein GIT1 antibody
      • ARF GTPase-activating protein GIT1 antibody
      • CAT 1 antibody
      • CAT-1 antibody
      • CaT1 antibody
      • Cool associated and tyrosine phosphorylated protein 1 antibody
      • Cool-associated and tyrosine-phosphorylated protein 1 antibody
      • G protein coupled receptor kinase interacting ArfGAP 1 antibody
      • G protein coupled receptor kinase interactor 1 antibody
      • G protein-coupled receptor kinase-interactor 1 antibody
      • GIT1 antibody
      • GIT1_HUMAN antibody
      • GRK interacting protein 1 antibody
      • GRK-interacting protein 1 antibody
      see all

    Images

    • All lanes : Anti-GIT1 antibody [EPR10368] (ab171956) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : HeLa lysate
      Lane 3 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 84 kDa

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GIT1 using ab171956 at 1/100 dilution

    • Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling GIT1 using ab171956 at 1/100 dilution

    References

    ab171956 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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