Overview

  • Product name
  • Description
    Rabbit polyclonal to GITR
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human GITR aa 90-162.
    Database link: Q9Y5U5

  • Positive control
    • IHC-P: Human cervical cancer and lymph node tissue. ICC/IF: HepG2 cells.
  • General notes

     This product was previously labelled as TNFRSF18

     

Properties

Applications

Our Abpromise guarantee covers the use of ab236254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/500 - 1/1000.

Target

  • Function
    Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
  • Tissue specificity
    Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
  • Sequence similarities
    Contains 3 TNFR-Cys repeats.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducible TNFR family receptor antibody
    • Activation-inducible TNFR family receptor antibody
    • AITR antibody
    • CD357 antibody
    • GITR antibody
    • GITR D antibody
    • GITR-D antibody
    • GITRD antibody
    • Glucocorticoid induced TNFR related protein antibody
    • glucocorticoid-induced tnf receptor ligand antibody
    • glucocorticoid-induced tnf receptors ligand antibody
    • Glucocorticoid-induced TNFR-related protein antibody
    • TNF receptor superfamily activation inducible protein antibody
    • TNFRSF 18 antibody
    • TNFRSF18 antibody
    • TNR18_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 18 antibody
    • Tumor necrosis factor receptor superfamily member 18 precursor antibody
    see all

Images

  • HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for GITR using ab236254 at a dilution of 1/166 in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L) (green). Counterstained with DAPI (blue).

    The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C.

  • Paraffin-embedded human lymph node tissue stained for GITR with ab236254 at a 1/500 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Paraffin-embedded human cervical cancer tissue stained for GITR with ab236254 at a 1/500 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

References

ab236254 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236254.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up