Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CAL61] to GITR
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse
Product nameAnti-GITR antibody [CAL61]
See all GITR primary antibodies
DescriptionRabbit monoclonal [CAL61] to GITR
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse
Synthetic peptide within Mouse GITR aa 175-228. The exact sequence is proprietary.
Database link: O35714
- IHC-P: Mouse lung and spleen tissue. WB: Mouse splenocytes treated with concanavalin A. Flow Cyt: Mouse splenocytes treated with concanavalin A.
This product was previously labeled as TNFRSF18.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.50
Preservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesPurity >99%
Our Abpromise guarantee covers the use of ab237725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 26 kDa.|
|IHC-P||1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionReceptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
Tissue specificityExpressed in lymph node, peripheral blood leukocytes and weakly in spleen.
Sequence similaritiesContains 3 TNFR-Cys repeats.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- Activation inducible TNFR family receptor antibody
- Activation-inducible TNFR family receptor antibody
- AITR antibody
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse lung is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse spleen is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
All lanes : Anti-GITR antibody [CAL61] (ab237725) at 1/1000 dilution
Lane 1 : Untreated mouse splenocytes whole cell lysate
Lane 2 : Mouse splenocytes treated with 2.5µg/ml concanavalin A for 3 days whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 26 kDa
Exposure time: 3 minutes
33 kDa band represents GITR isoform B based on Uniprot and literature. (PMID: 10836847).
Blocking/Dilution buffer: 5% NFDM/TBST.
Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days labeling GITR with ab237725 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab237725 (Right).
Formalin-fixed, paraffin-embedded mouse spleen tissue stained for GITR using ab237725 at 0.125 µg/ml dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237725 has not yet been referenced specifically in any publications.