Recombinant

Recombinant Anti-GITR antibody [CAL61] - BSA and Azide free (ab251610)

Overview

  • Product name
    Anti-GITR antibody [CAL61] - BSA and Azide free
    See all GITR primary antibodies
  • Description
    Rabbit monoclonal [CAL61] to GITR - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse GITR aa 175-228. The exact sequence is proprietary.
    Database link: O35714

  • Positive control
    • IHC-P: Mouse lung and spleen tissue. WB: Mouse splenocytes treated with concanavalin A. Flow Cyt: Mouse splenocytes treated with concanavalin A.
  • General notes

    ab251610 is a PBS-only buffer format of ab237725. Please refer to ab237725 for recommended dilutions, protocols, and image data.

     This product was previously labeled as TNFRSF18.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab251610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function
      Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
    • Tissue specificity
      Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
    • Sequence similarities
      Contains 3 TNFR-Cys repeats.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activation inducible TNFR family receptor antibody
      • Activation-inducible TNFR family receptor antibody
      • AITR antibody
      • CD357 antibody
      • GITR antibody
      • GITR D antibody
      • GITR-D antibody
      • GITRD antibody
      • Glucocorticoid induced TNFR related protein antibody
      • glucocorticoid-induced tnf receptor ligand antibody
      • glucocorticoid-induced tnf receptors ligand antibody
      • Glucocorticoid-induced TNFR-related protein antibody
      • TNF receptor superfamily activation inducible protein antibody
      • TNFRSF 18 antibody
      • TNFRSF18 antibody
      • TNR18_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 18 antibody
      • Tumor necrosis factor receptor superfamily member 18 precursor antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse lung is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237725).

    • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GITR with ab237725 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the mouse spleen is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237725).

    • Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes treated with 2.5μg/ml concanavalin A for 3 days labeling GITR with ab237725 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellularly stained with rabbit IgG (Left) or ab237725 (Right). 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237725).

    • Formalin-fixed, paraffin-embedded mouse spleen tissue stained for GITR using ab237725 at 0.125 µg/ml dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237725).

    References

    ab251610 has not yet been referenced specifically in any publications.

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