Recombinant

Recombinant Anti-GITR antibody [CAL8] - BSA and Azide free (ab251601)

Overview

  • Product name
    Anti-GITR antibody [CAL8] - BSA and Azide free
    See all GITR primary antibodies
  • Description
    Rabbit monoclonal [CAL8] to GITR - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-Pmore details
    Unsuitable for: ICC/IF,IP or WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human GITR aa 150 to the C-terminus.
    Database link: Q9Y5U5

  • Positive control
    • IHC-P: Human tonsil tissue. Flow Cyt: Human PBMCs.
  • General notes

    ab251601 is a PBS-only buffer format of ab237714. Please refer to ab237714 for recommended dilutions, protocols, and image data.

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product was previously labeled as TNFRSF18.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab251601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF,IP or WB.
  • Target

    • Function
      Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
    • Tissue specificity
      Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
    • Sequence similarities
      Contains 3 TNFR-Cys repeats.
    • Cellular localization
      Secreted and Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activation inducible TNFR family receptor antibody
      • Activation-inducible TNFR family receptor antibody
      • AITR antibody
      • CD357 antibody
      • GITR antibody
      • GITR D antibody
      • GITR-D antibody
      • GITRD antibody
      • Glucocorticoid induced TNFR related protein antibody
      • glucocorticoid-induced tnf receptor ligand antibody
      • glucocorticoid-induced tnf receptors ligand antibody
      • Glucocorticoid-induced TNFR-related protein antibody
      • TNF receptor superfamily activation inducible protein antibody
      • TNFRSF 18 antibody
      • TNFRSF18 antibody
      • TNR18_HUMAN antibody
      • Tumor necrosis factor receptor superfamily member 18 antibody
      • Tumor necrosis factor receptor superfamily member 18 precursor antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling GITR with ab237714 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human tonsil tissue is observed. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237714).

    • Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) treated with 10μg/ml PHA for 48hrs, labeling GITR with ab237714 at 1/500 (right) compared with a rabbit IgG (left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/5000 dilution was used as the secondary antibody. Cells were surface stained with anti-CD25 conjugated to BV421. Then fixed with 2% PFA followed by intracellular staining rabbit IgG (Left) or ab237714 (Right).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab237714).

    References

    ab251601 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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