Overview

  • Product name
    Anti-GITR antibody [EPR20566]
    See all GITR primary antibodies
  • Description
    Rabbit monoclonal [EPR20566] to GITR
  • Host species
    Rabbit
  • Specificity
    We found ab223841 worked well in WB with recombinant protein only. The glycosylation of the endogenous target resulted in different molecular masses on the blot.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GITR aa 50-150. The exact sequence is proprietary.
    Database link: Q9Y5U5

  • Positive control
    • WB: HEK-293T transfected with human GITR, containing a Myc-GFP-tag, whole cell lysate. IHC-P: Human tonsil, colon and Hodgkin's lymphoma tissues. ICC/IF: HEK-293T cells transfected with GFP-tagged GITR expression vector.
  • General notes

     This product was previously labelled as TNFRSF18

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 26 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Function
    Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
  • Tissue specificity
    Expressed in lymph node, peripheral blood leukocytes and weakly in spleen.
  • Sequence similarities
    Contains 3 TNFR-Cys repeats.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activation inducible TNFR family receptor antibody
    • Activation-inducible TNFR family receptor antibody
    • AITR antibody
    • CD357 antibody
    • GITR antibody
    • GITR D antibody
    • GITR-D antibody
    • GITRD antibody
    • Glucocorticoid induced TNFR related protein antibody
    • glucocorticoid-induced tnf receptor ligand antibody
    • glucocorticoid-induced tnf receptors ligand antibody
    • Glucocorticoid-induced TNFR-related protein antibody
    • TNF receptor superfamily activation inducible protein antibody
    • TNFRSF 18 antibody
    • TNFRSF18 antibody
    • TNR18_HUMAN antibody
    • Tumor necrosis factor receptor superfamily member 18 antibody
    • Tumor necrosis factor receptor superfamily member 18 precursor antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling GITR with ab223841 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in sporadic cells (arrows) in human Hodgkin’s lymphoma (PMID: 25787078; PMID: 23785514). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells, transfected with GFP only or with GFP-tagged GITR expression vector, labeling GITR with ab223841 at 1/500 dilution followed by by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) secondary antibody (white) at 1/500 dilution. Confocal image showing cytoplasmic staining in HEK-293T cells transfected with GFP-tagged GITR expression vector.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079) secondary antibody at 1/500 dilution.

  • All lanes : Anti-GITR antibody [EPR20566] (ab223841) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with an empty vector (vector control) containing a Myc-GFP-tag whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with human GITR, containing a Myc-GFP-tag, whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 52 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling GITR with ab223841 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in stromal cells (arrows) in human colon (PMID: 25787078; PMID: 23785514). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling GITR using ab223841 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in sporadic cells (arrows) in human tonsil (PMID: 25787078; PMID: 23785514). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

References

ab223841 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab223841.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up