Overview

  • Product name

  • Description

    Rabbit polyclonal to GIV
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human GIV conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Human tonsil tissue, Rat brain tissue lysate. IF/ICC: Malme-3M cell line.
  • General notes

    Previously labelled as Girdin. 

Properties

Applications

Our Abpromise guarantee covers the use of ab113890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 216 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome.
  • Tissue specificity

    Expressed ubiquitously.
  • Sequence similarities

    Belongs to the CCDC88 family.
  • Post-translational
    modifications

    Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration.
  • Cellular localization

    Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides.
  • Information by UniProt
  • Database links

  • Alternative names

    • AKT iphosphorylation enhancer antibody
    • Akt phosphorylation enhancer antibody
    • APE antibody
    • Ccdc88a antibody
    • Coiled coil domain containing 88A antibody
    • Coiled coil domain containing protein 88A antibody
    • Coiled-coil domain-containing protein 88A antibody
    • FLJ10392 antibody
    • G alpha interacting vesicle associated protein antibody
    • G alpha-interacting vesicle-associated protein antibody
    • Galpha interacting vesicle associated protein antibody
    • Girders of actin filament antibody
    • Girdin antibody
    • GIV antibody
    • GRDN antibody
    • GRDN_HUMAN antibody
    • HkRP1 antibody
    • Hook related protein 1 antibody
    • Hook-related protein 1 antibody
    • KIAA1212 antibody
    see all

Images

  • Lane 1 : Anti-GIV antibody (ab113890) at 1 µg/ml
    Lane 2 : Anti-GIV antibody (ab113890) at 2 µg/ml

    All lanes : Rat brain lysate

    Predicted band size: 216 kDa

  • ab113890, at 5 µg/ml, staining GIV in Formalin-Fixed, Paraffin-Embedded Human tonsil tissue by immunohistochemistry.

  • ICC/IF image of ab113890 stained Malme-3M cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab113890, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Yang Z  et al. Girdin protein: A potential metastasis predictor associated with prognosis in lung cancer. Exp Ther Med 15:2837-2843 (2018). Read more (PubMed: 29456687) »
See 1 Publication for this product

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