Overview

  • Product name

    Anti-GIV antibody [EPR18433]
    See all GIV primary antibodies
  • Description

    Rabbit monoclonal [EPR18433] to GIV
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human GIV aa 200-400. The exact sequence is proprietary.
    Database link: Q3V6T2

  • Positive control

    • WB: HCT 116, HeLa, C6, RAW 264.7 and PC-12 whole cell lysates; Human fetal kidney, mouse brain and rat brain lysates. ICC/IF: HeLa and NIH/3T3 cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.
  • General notes

    Previously labelled as Girdin.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 208-216 kDa (predicted molecular weight: 216 kDa).
ICC/IF 1/2000.
Flow Cyt 1/150.
IP 1/50.

Target

  • Function

    Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome.
  • Tissue specificity

    Expressed ubiquitously.
  • Sequence similarities

    Belongs to the CCDC88 family.
  • Post-translational
    modifications

    Phosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration.
  • Cellular localization

    Membrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides.
  • Information by UniProt
  • Database links

  • Alternative names

    • AKT iphosphorylation enhancer antibody
    • Akt phosphorylation enhancer antibody
    • APE antibody
    • Ccdc88a antibody
    • Coiled coil domain containing 88A antibody
    • Coiled coil domain containing protein 88A antibody
    • Coiled-coil domain-containing protein 88A antibody
    • FLJ10392 antibody
    • G alpha interacting vesicle associated protein antibody
    • G alpha-interacting vesicle-associated protein antibody
    • Galpha interacting vesicle associated protein antibody
    • Girders of actin filament antibody
    • Girdin antibody
    • GIV antibody
    • GRDN antibody
    • GRDN_HUMAN antibody
    • HkRP1 antibody
    • Hook related protein 1 antibody
    • Hook-related protein 1 antibody
    • KIAA1212 antibody
    see all

Images

  • Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 216 kDa
    Observed band size: 208-216 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID: 23195430; PMID: 22308453).

  • GIV was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab179481 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab179481 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
    Lane 1: HeLa whole cell lysate 10µg (Input).
    Lane 2: ab179481 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab179481 in HeLa whole cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 seconds.

  • Anti-GIV antibody [EPR18433] (ab179481) at 1/1000 dilution + Human fetal kidney lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 216 kDa
    Observed band size: 208-216 kDa why is the actual band size different from the predicted?


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID: 23195430; PMID: 22308453).

  • Anti-GIV antibody [EPR18433] (ab179481) at 1/1000 dilution + HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 216 kDa
    Observed band size: 208-216 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Multiple bands represent 5 isoforms ranging from 208 to 216 kDa (PMID: 23195430; PMID: 22308453).

  • All lanes : Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 216 kDa
    Observed band size: 208-216 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 30 seconds; Lane 2: 3 minutes.

    Based on UniProt annotation, mouse GIV has 3 isoforms (215, 212, 205 kDa).

  • All lanes : Anti-GIV antibody [EPR18433] (ab179481) at 1/5000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 216 kDa
    Observed band size: 208-216 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Based on UniProt annotation, mouse GIV has 3 isoforms (215, 212, 205KD).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling GIV with ab179481 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with  Anti-alpha Tubulin antibody-Loading Control  (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (AlexaFluor®594) preadsorbed (ab150120) at  1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab179481 at 1/2000 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077 at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling GIV with ab179481 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody-Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L  (AlexaFluor®594) preadsorbed (ab150120) at  1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab179481 at 1/2000 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291 at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling GIV with ab179481 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

References

This product has been referenced in:

  • Wang S  et al. Girdin regulates the proliferation and apoptosis of pancreatic cancer cells via the PI3K/Akt signalling pathway. Oncol Rep 40:599-608 (2018). Read more (PubMed: 29901184) »
  • Yu L  et al. Silencing the Girdin gene enhances radio-sensitivity of hepatocellular carcinoma via suppression of glycolytic metabolism. J Exp Clin Cancer Res 36:110 (2017). Read more (PubMed: 28810896) »
See all 2 Publications for this product

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