Key features and details
- Rabbit polyclonal to GIV (phospho S1417)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-GIV (phospho S1417) antibody
See all GIV primary antibodies
DescriptionRabbit polyclonal to GIV (phospho S1417)
Specificityab111440 detects endogenous levels of GIV only when phosphorylated at serine 1417.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human GIV. Around the phosphorylation site of serine 1417 (Q-K-SP-L-T).
- Human breast carcinoma tissue
Previously labelled as Girdin.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.88% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab111440 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab111440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Enhances phosphoinositide 3-kinase (PI3K)-dependent phosphorylation and kinase activity of AKT1/PKB, but does not possess kinase activity itself. Phosphorylation of AKT1/PKB thereby induces the phosphorylation of downstream effectors GSK3 and FOXO1/FKHR, and regulates DNA replication and cell proliferation (By similarity). Essential for the integrity of the actin cytoskeleton and for cell migration. Required for formation of actin stress fibers and lamellipodia. May be involved in membrane sorting in the early endosome.
Tissue specificityExpressed ubiquitously.
Sequence similaritiesBelongs to the CCDC88 family.
modificationsPhosphorylation is induced by epidermal growth factor (EGF) in a phosphoinositide 3-kinase (PI3K)-dependent manner. Phosphorylation by AKT1/PKB is necessary for the delocalization from the cell membrane and for cell migration.
Cellular localizationMembrane. Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Cell projection > lamellipodium. Localizes to the cell membrane through interaction with phosphoinositides.
- Information by UniProt
- AKT iphosphorylation enhancer antibody
- Akt phosphorylation enhancer antibody
- APE antibody
ab111440 has not yet been referenced specifically in any publications.