Overview

  • Product name
  • Description
    Rabbit polyclonal to GJB2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminal of human Connexin 26, different from the related mouse and rat sequences by two amino acids.

  • Positive control
    • WB: Rat liver lysate. IHC-P: Human colon tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
ICC Use a concentration of 1 - 2 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
  • Involvement in disease
    Defects in GJB2 are the cause of deafness autosomal recessive type 1A (DFNB1A) [MIM:220290]. DFNB1A is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
    Defects in GJB2 are the cause of deafness autosomal dominant type 3A (DFNA3A) [MIM:601544].
    Defects in GJB2 are a cause of Vohwinkel syndrome (VS) [MIM:124500]. VS is an autosomal dominant disease characterized by hyperkeratosis, constriction on finger and toes and congenital deafness.
    Defects in GJB2 are a cause of palmoplantar keratoderma with deafness (PPKDFN) [MIM:148350]. PPKDFN is an autosomal dominant disorder characterized by the association of palmoplantar hyperkeratosis with progressive, bilateral, high-frequency, sensorineural deafness.
    Defects in GJB2 are a cause of keratitis-ichthyosis-deafness syndrome (KID syndrome) [MIM:148210]; an autosomal dominant form of ectodermal dysplasia. Ectodermal dysplasias (EDs) constitute a heterogeneous group of developmental disorders affecting tissues of ectodermal origin. EDs are characterized by abnormal development of two or more ectodermal structures such as hair, teeth, nails and sweat glands, with or without any additional clinical sign. Each combination of clinical features represents a different type of ectodermal dysplasia. KID syndrome is characterized by the association of hyperkeratotic skin lesions with vascularizing keratitis and profound sensorineural hearing loss. Clinical features include deafness, ichthyosis, photobia, absent or decreased eyebrows, sparse or absent scalp hair, decreased sweating and dysplastic finger and toenails.
    Defects in GJB2 are the cause of Bart-Pumphrey syndrome (BPS) [MIM:149200]. BPS is an autosomal dominant disorder characterized by sensorineural hearing loss, palmoplantar keratoderma, knuckle pads, and leukonychia, It shows considerable phenotypic variability.
    Defects in GJB2 are the cause of ichthyosis hystrix-like with deafness syndrome (HID syndrome) [MIM:602540]. HID syndrome is an autosomal-dominant inherited keratinizing disorder characterized by sensorineural deafness and spiky hyperkeratosis affecting the entire skin. HID syndrome is considered to differ from the similar KID syndrome in the extent and time of occurrence of skin symptoms and the severity of the associated keratitis.
  • Sequence similarities
    Belongs to the connexin family. Beta-type (group I) subfamily.
  • Cellular localization
    Cell membrane. Cell junction > gap junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • connexin 26 antibody
    • Connexin-26 antibody
    • Cx26 antibody
    • CXB2_HUMAN antibody
    • DFNA3 antibody
    • DFNB1 antibody
    • Gap junction beta-2 protein antibody
    • GJB2 antibody
    • HID antibody
    • KID antibody
    • NSRD1 antibody
    • PPK antibody
    see all

Images

  • Anti-GJB2 antibody (ab65969) at 1 µg/ml + Rat liver lysate

    Predicted band size: 26 kDa
    Observed band size: 26 kDa

  • IHC image of ab65969 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65969, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Dovmark TH  et al. Connexin-43 channels are a pathway for discharging lactate from glycolytic pancreatic ductal adenocarcinoma cells. Oncogene 36:4538-4550 (2017). WB . Read more (PubMed: 28368405) »
  • Hulikova A  et al. Stromal uptake and transmission of acid is a pathway for venting cancer cell-generated acid. Proc Natl Acad Sci U S A 113:E5344-53 (2016). SDS-PAGE ; Human . Read more (PubMed: 27543333) »
See all 3 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
EnVision Flex Peroxidase-blocking reagent (ready-to-use - DAKO) as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Cow Tissue sections (Fetal Liver)
Specification
Fetal Liver
Permeabilization
No
Fixative
Formaldehyde

Dr. Juliana Shimara Pires Ferrão

Verified customer

Submitted Nov 24 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (theca)
Permeabilization
Yes - 0.05% tween-20
Specification
theca
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
4% neutral buffered formalin

Miss. Barbara Makela

Verified customer

Submitted Jul 30 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (aglandular stomach, epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
aglandular stomach, epithelium
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methacarn

F Hernandez-Blazquez

Verified customer

Submitted Mar 16 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Intestine)
Specification
Intestine
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Protein Block, Serum-Free - Dako as blocking agent for 30 minute(s) · Concentration: 0.25%

Abcam user community

Verified customer

Submitted Mar 29 2013

Answer

Thank you for contacting us and sorry for the delay in getting back to you.

The immunogen used to raise the Anti-GJB2 antibody (ab65969) is actually raised against a peptide taken from the N-terminal end of human GJB2. Not the middle region of rat GFB2 as currently stated on the datasheet. Unfortunately this information was entered in error. I am sorry for this and any inconvenience caused. This information will be updated as soon as possible.

The antibody is raised against a peptide taken from a cytoplasmic region and would therefore not bind the extracellular domain. I have looked through the alternative antibodies we have against this target and have not been able to find one which would be likely to detect the extracellular domain.

I am sorry that I could not be of more help in this instance. If you have any further questions, please do not hesitate to ask.

Read More

Answer

Thank you for contacting us.

As your customer is running a sample of non-transfected HeLa cells as well, the absence of the 18 kDa band in this lane indicates that it is only an artefact due to the transfection. If it would be a cross-reactivity with another endogenous protein, the non-transfected HeLa cells should show this as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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