Overview

  • Product name

  • Description

    Rabbit polyclonal to GK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-302 of Human GK2 (Uniprot ID: Q14410).

  • Positive control

    • HepG2 whole cell lysate; Human OVCA xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Relevance

    GK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism. It is a phosphotransferase enzyme involved in lipolysis and catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol phosphate. This protein could be the product of a pseudogene.
  • Cellular localization

    Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By similarity). In sperm the majority of the enzyme is bound to mitochondria.
  • Database links

  • Alternative names

    • ATP:glycerol 3 phosphotransferase antibody
    • EC 2.7.1.30 antibody
    • GK 2 antibody
    • GK antibody
    • GKP2 antibody
    • GKTA antibody
    • Glycerokinase antibody
    • Glycerol kinase 2 antibody
    • Glycerol kinase testis specific 2 antibody
    see all

Images

  • Anti-GK2 antibody (ab153926) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue, labeling GK2 with ab153926 at 1/100 dilution.

References

ab153926 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab153926.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up