Anti-GK2 antibody (ab153926)

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Overview

  • Product name

    Anti-GK2 antibody
    See all GK2 primary antibodies

  • Description

    Rabbit polyclonal to GK2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-302 of Human GK2 (Uniprot ID: Q14410).

  • Positive control

    • HepG2 whole cell lysate; Human OVCA xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab153926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Relevance

    GK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism. It is a phosphotransferase enzyme involved in lipolysis and catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol phosphate. This protein could be the product of a pseudogene.

  • Cellular localization

    Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By similarity). In sperm the majority of the enzyme is bound to mitochondria.

  • Database links

    see all

  • Alternative names

    • ATP:glycerol 3 phosphotransferase antibody
    • EC 2.7.1.30 antibody
    • GK 2 antibody
    • GK antibody
    • GKP2 antibody
    • GKTA antibody
    • Glycerokinase antibody
    • Glycerol kinase 2 antibody
    • Glycerol kinase testis specific 2 antibody
    see all

Images

  • Western blot - Anti-GK2 antibody (ab153926)
    Western blot - Anti-GK2 antibody (ab153926)
    Anti-GK2 antibody (ab153926) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GK2 antibody (ab153926)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GK2 antibody (ab153926)
    Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue, labeling GK2 with ab153926 at 1/100 dilution.

References

ab153926 has not yet been referenced specifically in any publications.

Publishing research using ab153926? Please let us know so that we can cite the reference in this datasheet.

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