Anti-GK2 antibody (ab153926)

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Key features and details

  • Rabbit polyclonal to GK2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-GK2 antibody
    See all GK2 primary antibodies

  • Description

    Rabbit polyclonal to GK2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey

  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-302 of Human GK2 (Uniprot ID: Q14410).

  • Positive control

    • HepG2 whole cell lysate; Human OVCA xenograft tissue.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab153926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).

Target

  • Relevance

    GK2 (glycerol kinase 2) is a key enzyme in the regulation of glycerol uptake and metabolism. It is a phosphotransferase enzyme involved in lipolysis and catalyzes the transfer of a phosphate from ATP to glycerol thus forming glycerol phosphate. This protein could be the product of a pseudogene.

  • Cellular localization

    Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side (By similarity). Cytoplasm (By similarity). In sperm the majority of the enzyme is bound to mitochondria.

  • Database links

    see all

  • Alternative names

    • ATP:glycerol 3 phosphotransferase antibody
    • EC 2.7.1.30 antibody
    • GK 2 antibody
    • GK antibody
    • GKP2 antibody
    • GKTA antibody
    • Glycerokinase antibody
    • Glycerol kinase 2 antibody
    • Glycerol kinase testis specific 2 antibody
    see all

Images

  • Western blot - Anti-GK2 antibody (ab153926)
    Western blot - Anti-GK2 antibody (ab153926)
    Anti-GK2 antibody (ab153926) at 1/500 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 61 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GK2 antibody (ab153926)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GK2 antibody (ab153926)
    Immunohistochemical analysis of paraffin-embedded Human OVCA xenograft tissue, labeling GK2 with ab153926 at 1/100 dilution.

References (0)

Publishing research using ab153926? Please let us know so that we can cite the reference in this datasheet.

ab153926 has not yet been referenced specifically in any publications.

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