Product nameAnti-GKAP1 antibody
DescriptionRabbit polyclonal to GKAP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Elephant
Synthetic peptide, corresponding to a region between N terminal amino acids 1-50 of Human GKAP1 (NP_079487.2).
- 293T, HeLa and Jurkat whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab123594 was affinity purified using an epitope specific to GKAP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab123594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
Sequence similaritiesBelongs to the GKAP1 family.
Cellular localizationGolgi apparatus.
- Information by UniProt
- cGMP dependent protein kinase anchoring protein 42kDa antibody
- cGMP dependent protein kinase anchoring protein of 42 kDa antibody
- cGMP-dependent protein kinase-anchoring protein of 42 kDa antibody
All lanes : Anti-GKAP1 antibody (ab123594) at 0.1 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 10 seconds
Detection of GKAP1 by Western Blot of Immunprecipitate.
ab123594 at 1µg/ml staining GKAP1 in 293T whole cell lysate immunoprecipitated using ab123594 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
ab123594 has not yet been referenced specifically in any publications.