Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15376(B)] to GKN2
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-GKN2 antibody [EPR15376(B)]
See all GKN2 primary antibodies
DescriptionRabbit monoclonal [EPR15376(B)] to GKN2
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human GKN2 aa 1-100. The exact sequence is proprietary.
Database link: Q86XP6
- Human stomach tissue and lysates.
This product was previously labelled as Gastrokine 2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab184163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|IP||1/60 - 1/80.|
Tissue specificityExpressed in gastric mucosa.
Sequence similaritiesContains 1 BRICHOS domain.
- Information by UniProt
- BLOT antibody
- Blottin antibody
- BRICD1B antibody
Anti-GKN2 antibody [EPR15376(B)] (ab184163) at 1/50000 dilution + Human stomach lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 20 kDa
The observed MW is lower than the predicted 20kDa, which may be due to the removing of a 20 amino acid signal peptide.
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab184163 at 1/500 dilution. The slide is counterstained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation of Human stomach lysates (lane 1) or PBS (lane 2) using ab184163. Detection of GKN2 utilised ab184163 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.
ab184163 has not yet been referenced specifically in any publications.