Recombinant
RabMAb

Recombinant Anti-GKN2 antibody [EPR15376(B)] (ab184163)

Overview

  • Product name

    Anti-GKN2 antibody [EPR15376(B)]
    See all GKN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15376(B)] to GKN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GKN2 aa 1-100. The exact sequence is proprietary.
    Database link: Q86XP6

  • Positive control

    • Human stomach tissue and lysates.
  • General notes

     

     

     This product was previously labelled as Gastrokine 2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15376(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184163 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/250 - 1/500.
IP 1/60 - 1/80.

Target

  • Tissue specificity

    Expressed in gastric mucosa.
  • Sequence similarities

    Contains 1 BRICHOS domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • BLOT antibody
    • Blottin antibody
    • BRICD1B antibody
    • BRICHOS domain containing 1B antibody
    • Down regulated in gastric cancer GDDR antibody
    • Down-regulated in gastric cancer antibody
    • Gastrokine-2 antibody
    • GDDR antibody
    • Gkn2 antibody
    • GKN2_HUMAN antibody
    • PRO813 antibody
    • TFIZ1 antibody
    • Trefoil factor interactions(z) 1 antibody
    • UNQ465/PRO813 antibody
    • VLTI465 antibody
    see all

Images

  • Anti-GKN2 antibody [EPR15376(B)] (ab184163) at 1/50000 dilution + Human stomach lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    The observed MW is lower than the predicted 20kDa, which may be due to the removing of a 20 amino acid signal peptide.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab184163 at 1/500 dilution. The slide is counterstained with Hematoxylin.

  • Immunoprecipitation of Human stomach lysates (lane 1) or PBS (lane 2) using ab184163. Detection of GKN2 utilised ab184163 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution.

     

     

References

ab184163 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab184163.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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