Recombinant
RabMAb

Recombinant Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866)

Overview

  • Product name

    Anti-GKN2 antibody [EPR15377(B)] - C-terminal
    See all GKN2 primary antibodies
  • Description

    Rabbit monoclonal [EPR15377(B)] to GKN2 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human GKN2 aa 100 to the C-terminus. The exact sequence is proprietary.
    Database link: Q86XP6

  • Positive control

    • Human, mouse and rat stomach lysates; Mouse and rat stomach tissues.
  • General notes

    This product was previously labelled as Gastrokine 2.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15377(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab188866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000 - 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/50.
WB 1/1000 - 1/2000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).

Target

Images

  • All lanes : Anti-GKN2 antibody [EPR15377(B)] - C-terminal (ab188866) at 1/5000 dilution

    Lane 1 : Human stomach lysate
    Lane 2 : Mouse stomach lysate
    Lane 3 : Rat stomach lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 20 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling GKN2 with ab188866 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling GKN2 with ab188866 at 1/4,000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot analysis of GKN2 in Human stomach lysate immunoprecipitated using ab188866 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

References

ab188866 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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