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    glb1beta-galactosidase-antibody-ab96239.pdf

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Anti-GLB1/Beta-galactosidase antibody (ab96239)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (3)

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Western blot - Anti-GLB1/Beta-galactosidase antibody (ab96239)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody (ab96239)

Key features and details

  • Rabbit polyclonal to GLB1/Beta-galactosidase
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Recombinant Human GLB1/Beta-galactosidase protein (ab151890)

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Overview

  • Product name

    Anti-GLB1/Beta-galactosidase antibody
    See all GLB1/Beta-galactosidase primary antibodies
  • Description

    Rabbit polyclonal to GLB1/Beta-galactosidase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human GLB1/Beta-galactosidase.

  • Positive control

    • H1299 cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human GLB1/Beta-galactosidase protein (ab151890)

Applications

Our Abpromise guarantee covers the use of ab96239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 76 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

Target

  • Function

    Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.
    Isoform 2 has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.
  • Involvement in disease

    Defects in GLB1 are the cause of GM1-gangliosidosis type 1 (GM1G1) [MIM:230500]; also known as infantile GM1-gangliosidosis. GM1-gangliosidosis is an autosomal recessive lysosomal storage disease marked by the accumulation of GM1 gangliosides, glycoproteins and keratan sulfate primarily in neurons of the central nervous system. GM1G1 is characterized by onset within the first three months of life, central nervous system degeneration, coarse facial features, hepatosplenomegaly, skeletal dysmorphology reminiscent of Hurler syndrome, and rapidly progressive psychomotor deterioration. Urinary oligosaccharide levels are high. It leads to death usually between the first and second year of life.
    Defects in GLB1 are the cause of GM1-gangliosidosis type 2 (GM1G2) [MIM:230600]; also known as late infantile/juvenile GM1-gangliosidosis. GM1G2 is characterized by onset between ages 1 and 5. The main symptom is locomotor ataxia, ultimately leading to a state of decerebration with epileptic seizures. Patients do not display the skeletal changes associated with the infantile form, but they nonetheless excrete elevated amounts of beta-linked galactose-terminal oligosaccharides. Inheritance is autosomal recessive.
    Defects in GLB1 are the cause of GM1-gangliosidosis type 3 (GM1G3) [MIM:230650]; also known as adult or chronic GM1-gangliosidosis. GM1G3 is characterized by a variable phenotype. Patients show mild skeletal abnormalities, dysarthria, gait disturbance, dystonia and visual impairment. Visceromegaly is absent. Intellectual deficit can initially be mild or absent but progresses over time. Inheritance is autosomal recessive.
    Defects in GLB1 are the cause of mucopolysaccharidosis type 4B (MPS4B) [MIM:253010]; also known as Morquio syndrome B. MPS4B is a form of mucopolysaccharidosis type 4, an autosomal recessive lysosomal storage disease characterized by intracellular accumulation of keratan sulfate and chondroitin-6-sulfate. Key clinical features include short stature, skeletal dysplasia, dental anomalies, and corneal clouding. Intelligence is normal and there is no direct central nervous system involvement, although the skeletal changes may result in neurologic complications. There is variable severity, but patients with the severe phenotype usually do not survive past the second or third decade of life.
  • Sequence similarities

    Belongs to the glycosyl hydrolase 35 family.
  • Cellular localization

    Lysosome and Cytoplasm > perinuclear region. Localized to the perinuclear area of the cytoplasm but not to lysosomes.
  • Target information above from: UniProt accession P16278 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2720 Human
    • Omim: 611458 Human
    • SwissProt: P16278 Human
    • Unigene: 443031 Human
    • Alternative names

      • Acid beta galactosidase antibody
      • Acid beta-galactosidase antibody
      • Beta galactosidase 1 antibody
      • Beta galactosidase antibody
      • Beta-galactosidase antibody
      • BGAL_HUMAN antibody
      • EBP antibody
      • EBP, included antibody
      • Elastin receptor 1 (67kD) antibody
      • Elastin receptor 1 67kDa antibody
      • Elastin receptor 1 antibody
      • Elastin receptor 1, included antibody
      • Elastin-binding protein, included antibody
      • ELNR1 antibody
      • Galactosidase beta 1 antibody
      • GLB 1 antibody
      • GLB1 antibody
      • Lactase antibody
      • MPS4B antibody
      • S-GAL, included antibody
      see all

    Images

    • Western blot - Anti-GLB1/Beta-galactosidase antibody (ab96239)
      Western blot - Anti-GLB1/Beta-galactosidase antibody (ab96239)
      Anti-GLB1/Beta-galactosidase antibody (ab96239) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

      Predicted band size: 76 kDa



      7.5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody (ab96239)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLB1/Beta-galactosidase antibody (ab96239)

      ab96239 staining GLB1/Beta-galactosidase in human ovarian carcinoma by immunohistochemical analysis.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (3)

    Publishing research using ab96239? Please let us know so that we can cite the reference in this datasheet.

    ab96239 has been referenced in 3 publications.

    • Mills TA  et al. Parathyroid Hormone-Related Peptide-Linked Hypercalcemia in a Melanoma Patient Treated With Ipilimumab: Hormone Source and Clinical and Metabolic Correlates. Semin Oncol 42:909-14 (2015). PubMed: 26615135
    • Martinez-Outschoorn UE  et al. Reverse Warburg effect in a patient with aggressive B-cell lymphoma: is lactic acidosis a paraneoplastic syndrome? Semin Oncol 40:403-18 (2013). PubMed: 23972703
    • Capparelli C  et al. Autophagy and senescence in cancer-associated fibroblasts metabolically supports tumor growth and metastasis via glycolysis and ketone production. Cell Cycle 11:2285-302 (2012). IHC-P ; Human . PubMed: 22684298

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-GLB1/Beta-galactosidase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Fixative
    Methanol
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 10 2018

    Question


    Thank you very much. Really I need this antibody for IHC-P, so really i want to buy it just i want to buy it for IHC. So, please is this paper can guarantee its use for IHC

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2012

    Answer

    Thank you for your reply.

    Yes, we would guarantee this antibody in IHC-P since it has been published to work. I hope this information helps. Please contact us with any other questions.

    Read More

    Abcam Scientific Support

    Answered on Jul 24 2012

    Question


    I would like your kind help.
    I read one paper published last month regarding the use of Ab-96239 antibody in paraffin -embedded human samples using immunohistochemistry and DAB reaction.
    However, when i opened the web site of ABCAM, i found that this antibody can be used only for western blot.
    Do you have any comment?
    The paper is published by Lisanti et al. in cell cycle 11:12,2285-2302, June 2012
    Thank you in advance

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2012

    Answer

    Thank you for contacting us.

    We do not test this antibody in IHC-P in house, only in WB. I've forwarded the publication to our references team to review. This is the first we've heard of ab96239 being used in IHC-P, but we may add this to our datasheet. Thank you for alerting us.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Jul 24 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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