Overview

  • Product name
  • Description
    Rabbit Polyclonal to GLCCI1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human GLCCI1 aa 233-263 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q86VQ1

  • Positive control
    • Human testis tissue; Human placenta tissue lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171137 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 58 kDa.

Target

Images

  • Anti-GLCCI1 antibody (ab171137) at 1/100 dilution + Human placenta tissue lysates at 35 µg

    Predicted band size: 58 kDa

  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human testis tissue, labeling GLCCI1 with ab171137 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

This product has been referenced in:
  • Hamada AM  et al. Association Between GLCCI1 Promoter Polymorphism (Rs37972) and Post-Transplant Hypertension in Renal Transplant Recipients. Kidney Blood Press Res 42:1155-1163 (2017). IHC-P ; Human . Read more (PubMed: 29224020) »
See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - Inactivation of endogeous peroxidases
Specification
Kidney
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

aki hamada

Verified customer

Submitted Jan 12 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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