Anti-Glycine decarboxylase antibody (ab97625)
Key features and details
- Rabbit polyclonal to Glycine decarboxylase
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glycine decarboxylase antibody
See all Glycine decarboxylase primary antibodies -
Description
Rabbit polyclonal to Glycine decarboxylase -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human Glycine decarboxylase aa 492-693.
Database link: NP_000161 -
Positive control
- HepG2 cells and lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97625 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 113 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 113 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein. -
Involvement in disease
Defects in GLDC are a cause of non-ketotic hyperglycinemia (NKH) [MIM:605899]; also known as glycine encephalopathy (GCE). NKH is an autosomal recessive disease characterized by accumulation of a large amount of glycine in body fluid and by severe neurological symptoms. -
Sequence similarities
Belongs to the gcvP family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 2731 Human
- Entrez Gene: 104174 Mouse
- Omim: 238300 Human
- SwissProt: P23378 Human
- SwissProt: Q91W43 Mouse
- Unigene: 584238 Human
- Unigene: 274852 Mouse
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Alternative names
- GCE antibody
- GCSP antibody
- GCSP_HUMAN antibody
see all
Images
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All lanes : Anti-Glycine decarboxylase antibody (ab97625) at 1/500 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 113 kDa5% SDS-PAGE
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ab97625, at a 1/200 dilution, staining Glycine decarboxylase in methanol fixed HeLa cells by Immunofluorescence analysis. Lower image was co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab97625 has been referenced in 5 publications.
- Xie H et al. GLDC mitigated by miR-30e regulates cell proliferation and tumor immune infiltration in TNBC. Front Immunol 13:1033367 (2022). PubMed: 36275705
- Zhou J et al. Identification and characterization of GLDC as host susceptibility gene to severe influenza. EMBO Mol Med 11:N/A (2019). PubMed: 30498026
- Li S et al. Astrocytes in primary cultures express serine racemase, synthesize d-serine and acquire A1 reactive astrocyte features. Biochem Pharmacol 151:245-251 (2018). PubMed: 29305854
- Swanson MA et al. Biochemical and molecular predictors for prognosis in nonketotic hyperglycinemia. Ann Neurol 78:606-18 (2015). PubMed: 26179960
- Kim SK et al. Differential expression of enzymes associated with serine/glycine metabolism in different breast cancer subtypes. PLoS One 9:e101004 (2014). WB ; Human . PubMed: 24979213